Inhibitory Potency of C-glycosyl Flavonoids from Morus sp. on Advanced Glycation End Products

Advanced glycation end products (AGEs) formation is associated with different pathophysiological conditions, including diabetes mellitus. Our study aimed at screening the effect of 80 % aqueous methanol extract of leaves (AQME) of three commercial varieties of Morus indica (G4, V1 and S36) and two wild varieties of Morus sp., (Morus laevigata - ML and Morus serrata-MS) on in vitro protein glycation in BSA-glucose model. G4 exhibited the highest AGEs inhibition and metal ion chelation. It also inhibited cross-linking of protein glycation and protein aggregation. The G4 extract had a total polyphenol content of 96 mg GAE g ^-1 and flavonoid content of 55.3 mg CE g ^-1 . C-glycosyl flavonoids were identified and quantified in G4 by UPLC/MS. This is the first study reporting the presence of luteolin (258 μg g ^-1 ) and apigenin (164 μg g ^-1 ) in G4 variety which possess the most active anti-glycation property. Thus, G4 could be protective against hyperglycemia-mediated protein damage.