Interaction of carbon nanotubes reinforced hydroxyapatite composite with Bacillus subtilis, P. aeruginosa and C. albicans

P. Khalid, M. A. Hussain, P. D. Sasi Rekha, C. Sanal, S. Suraj, M. Rajashekhar, V. B. Suman, Sangappa, A. B. Arun

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2 Citations (Scopus)


Hydroxyapatite (HA), as a bone mineral component, has been an attractive bioceramic for the reconstruction of hard tissues. However, its poor mechanical properties, including low fracture toughness and tensile strength, have been a significant challenge to the application of HA for the replacement of load-bearing and/or large bone defects. Hydroxyapatite (HA) composite is reinforced with high purity and well-functionalized Multiwalled Carbon Naotubes (MWCNT>98 wt%) having an average diameter of 15 nm. The cellular response of f-MWCNT, MWCNT-HA composites were examined to model gram positive and gram negative Bacteria B. subtilis, P. aeruginosa and yeast C. albicans. Ca(NO3)2.4H2O and (NH4)2HPO4 were used to synthesize HA in situ. MWCNT were functionalized by heating at 100°C in 3:1 ratio of H2SO4 and HNO3 for 60 m with stirring and dispersed in Sodium Dodecyl Benzene Sulphonate (SDBS) by sonication. Hydroxy Apatite (HA) particles were produced in MWCNTs solution by adding Ca(NO3)2.4H2O and (NH4)2HPO4 under vigorously stirring conditions. The composite were dried and washed in distilled water followed by heat treatment at 250°C to obtain CNT-HA powder. Using FTIR, FESEM and EDS does physicochemical characterization of the composite material. The interaction of f-MWCNT and MWCNT-HA were tested on Bacillus subtilis, P. aeruginosa and C. albicans. The zone of inhibition and MIC studies were carried out with a concentration range from 62.5-1000 μg/ml. The test result shows no zone of inhibition and MIC > 1000 μg/ml on bacteria and yeast. This result provides further evidence that the bio-nano interface can be developed for Carbon Nanotubes reinforced Hydroxyapatite composites for load-bearing bone implants, drug delivery and diagnostic applications.

Original languageEnglish
Pages (from-to)678-684
Number of pages7
JournalIndian Journal of Science and Technology
Issue number5
Publication statusPublished - 01-01-2014

All Science Journal Classification (ASJC) codes

  • General


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