Intraventricular methotrexate: Motor deficit in male Wistar rats

Sampath Madhyastha, S. N. Somayaji, K. Nalini, B. S. Nayak, K. L. Bairy

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Objective: To investigate the effect of intraventricular methotrexate on locomotor and exploratory activity in the male rats. Methods: Male Wistar rats aged 4 months were administered with 2 mg/kg and 3 mg/kg dose of methotrexate for 3 alternate days into the lateral ventricle through a cannula (implanted using stereotaxic apparatus). After a week, the locomotor and exploratory activity was assessed by open field test and dark/bright arena test including control groups. The dopamine level in the brainstem and frontal cortex was estimated by high pressure liquid chromatography. Results: Methotrexate at 2 mg/kg and 3 mg/kg dose altered locomotor and exploratory activity in both the test models used, along with a decrease in dopamine level in brainstem and frontal cortex. Conclusion: Intraventricular methotrexate produced motor deficit which is due to altered dopamine level in the brain. The severity of the effects depended on dose.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalIndian Journal of Pharmacology
Issue number5
Publication statusPublished - 10-2002

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)


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