INVESTIGATIONS ON CYTOTOXICITY OF IN-SITU SILVER-GOLD BIMETALLIC NANOPARTICLES

This study focuses on developing an energy-e"cient microelectro discharge machining (Micro-EDM) method to synthesize in situ-formed silver-gold (Ag-Au) bimetallic nanoparticles. Parametric investigations examined the effects of dielectric fluid temperature, voltage, current, pulse-on time, and multitool arrangements on particle morphology, concentration, stability, and distribution. Results showed that nanoparticles formed at 0°C were larger (18.20- 28.68 nm), while smaller sizes were achieved at 25°C (4.44-7.84 nm) and 50°C (5.72-6.35 nm). The work also includes the study of cytotoxicity using the MTT assay method against the A549 cancer cell line. The Ag-Au nanofluids exhibited anticancer activity, inhibiting cancer cells with an IC50 of 4:42μg/mL, achieving cell inhibition between 65% and 75% at varying silver concentrations.