Laser Doppler Flowmetry Recording for Rodent Stroke Model Confirmation

The assessment of cerebral blood flow (CBF) during cerebral and spinal ischemia and reperfusion with conventional methods such as hydrogen removal and autoradiography of antipyrine 14C is complex. However, laser Doppler flowmetry (LDF) is a non-invasive method for standard registration of local CBF in human and preclinical experimental animals. Transient focal cerebral ischemia will be induced in animals using a rodent middle cerebral artery occlusion (MCAO) model based on the arterial advancement of a nylon suture and reperfusion occurs after removal of the suture. The probe is placed and fixed with a fixation solution in the lateral flow and the local CBF was constantly monitored before after occlusion, ischemia, and reperfusion. The stroke experiment shows that a 50% fall from the baseline is recorded in LDF when the common carotid artery (CCA) is occluded and falls 80% from the baseline when the filament enters the middle cerebral artery region. The method is based on recording the Doppler shift in He-Ne monochromatic light from 2 mW (frequency or wavelength) to 632.8 nm, which is reflected in the flow of red blood cells. The technique uncovers a straight connection between relative changes in the Doppler sign and illness and pharmacological status, blood move through a wide scope of mind and spinal cord injuries. The main advantage of LDF is that it does not require penetration into the tissue. The main disadvantage of this method is that only a small part of the bloodstream needs to be recorded and the signal can be used arbitrarily instead of an absolute value. The flexibility and real-time recording of CBF may allow new insights into the alteration of small cerebral blood vessels.