TY - JOUR
T1 - Laser induced fluorescence spectroscopy analysis of kidney tissues
T2 - A pilot study for the identification of renal cell carcinoma
AU - Pavithran M, Sanoop
AU - Lukose, Jijo
AU - Barik, Bijay Kumar
AU - Periasami, Ammasi
AU - Kartha, V. B.
AU - Chawla, Arun
AU - Chidangil, Santhosh
N1 - Funding Information:
Authors are thankful to the Department of Science and Technology (DST), Govt. of India and Vision Group of Science and Technology (VGST), Govt. of Karnataka for the experimental facilities. Mr. Sanoop Pavithran M is thankful to Dr. TMA Pai Ph.D. fellowship. Authors are thankful to Mr. Vittal Shenoy and Mr. Udaya for the technical assistance in the Biophotonics laboratory. Authors acknowledge Dr. Guruprasad KP, Dr. Shama Prasada K and Ms. Divya Adiga from Manipal School of Life Sciences, Manipal Academy of Higher Education and Dr. Kanthilatha Pai from the Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, for their support.
Publisher Copyright:
© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
PY - 2023/11
Y1 - 2023/11
N2 - The 325 nm-excited autofluorescence spectra from cancerous and normal renal tissues were collected ex vivo biopsy tissue samples, through an optical fiber probe-based system. Noticeable changes in intensity/wavelength were observed in the fluorescence emissions from endogenous fluorophores such as collagen, Nicotinamide adenine dinucleotide (NADH), Vitamin A (retinol), and flavin adenine dinucleotide, in pathological conditions with respect to the normal state. The energy metabolism involved in clear cell renal cell carcinoma (ccRCC) and chromophobe renal cell carcinoma (chRCC) are reflected in the fluorescence emission band at 445 nm due to bound NADH attributed to enhanced oxidative phosphorylation in chRCC and emission at 465 nm contributed by free NADH showing higher glycolytic action in ccRCC. The principal component analysis and one-way ANOVA effectively discriminate ccRCC from chRCC. It is shown that laser induced fluorescence technique with 325 nm excitation can be a suitable technique for optical pathology and in vivo surgical boundary demarcation in renal cell carcinoma.
AB - The 325 nm-excited autofluorescence spectra from cancerous and normal renal tissues were collected ex vivo biopsy tissue samples, through an optical fiber probe-based system. Noticeable changes in intensity/wavelength were observed in the fluorescence emissions from endogenous fluorophores such as collagen, Nicotinamide adenine dinucleotide (NADH), Vitamin A (retinol), and flavin adenine dinucleotide, in pathological conditions with respect to the normal state. The energy metabolism involved in clear cell renal cell carcinoma (ccRCC) and chromophobe renal cell carcinoma (chRCC) are reflected in the fluorescence emission band at 445 nm due to bound NADH attributed to enhanced oxidative phosphorylation in chRCC and emission at 465 nm contributed by free NADH showing higher glycolytic action in ccRCC. The principal component analysis and one-way ANOVA effectively discriminate ccRCC from chRCC. It is shown that laser induced fluorescence technique with 325 nm excitation can be a suitable technique for optical pathology and in vivo surgical boundary demarcation in renal cell carcinoma.
UR - https://www.scopus.com/pages/publications/85169672947
UR - https://www.scopus.com/pages/publications/85169672947#tab=citedBy
U2 - 10.1002/jbio.202300021
DO - 10.1002/jbio.202300021
M3 - Article
AN - SCOPUS:85169672947
SN - 1864-063X
VL - 16
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 11
M1 - e202300021
ER -
