LC method for the estimation of Cefprozil in human plasma and its pharmacokinetics

Shivprakash; S. Reddy; B. Dasandi; C. Patel; K. M. Bhat

LC method for the estimation of Cefprozil in human plasma and its pharmacokinetics

Shivprakash, S. Reddy, B. Dasandi, C. Patel, K. M. Bhat

Research output: Contribution to journal › Article › peer-review

Abstract

A new method for the estimation of Cefprozil is developed and validated. This method is simple and rapid because the sample preparation is devoid of any solvent extraction. The plasma proteins were precipitated and the supernatant was directly injected to the HPLC system. The separation was achieved using RP 18e (5μ, 250 X 4 mm) column and the mobile phase was a mixture of Phosphate buffer and acetonitrile. The eluents monitored at 235nm. The column was maintained at a temperature of 40±0.1°C. The method was validated for sensitivity, specificity, precision and accuracy. The LOQ of this method is 0.25μg ml¹ and the calibration curve range is 0.25 to 30μg ml^-1. This method is used to study the comparative bioavailability of conventional and sustained release capsules of Cefprozil.

Original language	English
Pages (from-to)	86-90
Number of pages	5
Journal	Indian Drugs
Volume	41
Issue number	2
Publication status	Published - 01-02-2004

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science
Drug Discovery

Cite this

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abstract = "A new method for the estimation of Cefprozil is developed and validated. This method is simple and rapid because the sample preparation is devoid of any solvent extraction. The plasma proteins were precipitated and the supernatant was directly injected to the HPLC system. The separation was achieved using RP 18e (5μ, 250 X 4 mm) column and the mobile phase was a mixture of Phosphate buffer and acetonitrile. The eluents monitored at 235nm. The column was maintained at a temperature of 40±0.1°C. The method was validated for sensitivity, specificity, precision and accuracy. The LOQ of this method is 0.25μg ml1 and the calibration curve range is 0.25 to 30μg ml-1. This method is used to study the comparative bioavailability of conventional and sustained release capsules of Cefprozil.",

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AU - Shivprakash,

AU - Reddy, S.

AU - Dasandi, B.

AU - Patel, C.

AU - Bhat, K. M.

PY - 2004/2/1

Y1 - 2004/2/1

N2 - A new method for the estimation of Cefprozil is developed and validated. This method is simple and rapid because the sample preparation is devoid of any solvent extraction. The plasma proteins were precipitated and the supernatant was directly injected to the HPLC system. The separation was achieved using RP 18e (5μ, 250 X 4 mm) column and the mobile phase was a mixture of Phosphate buffer and acetonitrile. The eluents monitored at 235nm. The column was maintained at a temperature of 40±0.1°C. The method was validated for sensitivity, specificity, precision and accuracy. The LOQ of this method is 0.25μg ml1 and the calibration curve range is 0.25 to 30μg ml-1. This method is used to study the comparative bioavailability of conventional and sustained release capsules of Cefprozil.

AB - A new method for the estimation of Cefprozil is developed and validated. This method is simple and rapid because the sample preparation is devoid of any solvent extraction. The plasma proteins were precipitated and the supernatant was directly injected to the HPLC system. The separation was achieved using RP 18e (5μ, 250 X 4 mm) column and the mobile phase was a mixture of Phosphate buffer and acetonitrile. The eluents monitored at 235nm. The column was maintained at a temperature of 40±0.1°C. The method was validated for sensitivity, specificity, precision and accuracy. The LOQ of this method is 0.25μg ml1 and the calibration curve range is 0.25 to 30μg ml-1. This method is used to study the comparative bioavailability of conventional and sustained release capsules of Cefprozil.

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LC method for the estimation of Cefprozil in human plasma and its pharmacokinetics

Abstract

All Science Journal Classification (ASJC) codes

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