Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis

Kausalya Neelavara Makkithaya; Ming Che Chan; Wei Shiuan Tseng; Nirmal Mazumder; Guan Yu Zhuo

doi:10.1117/12.3097803

Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis

Kausalya Neelavara Makkithaya
, Ming Che Chan
, Wei Shiuan Tseng
, Nirmal Mazumder
, Guan Yu Zhuo^*

^*Corresponding author for this work

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate a novel approach combining two-photon microscopy with machine learning algorithms to analyze collagen organization in esophageal tissues, enabling differentiation between squamous cell carcinoma and high-grade dysplasia through quantitative extracellular matrix assessment.

Original language	English
Title of host publication	Translational Biophotonics
Subtitle of host publication	Diagnostics and Therapeutics IV
Editors	Zhiwei Huang, Lothar D. Lilge
Publisher	SPIE
ISBN (Electronic)	9781510698055
DOIs	https://doi.org/10.1117/12.3097803
Publication status	Published - 18-12-2025
Event	4th Translational Biophotonics: Diagnostics and Therapeutics - Munich, Germany Duration: 22-06-2025 → 26-06-2025

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	13934
ISSN (Print)	1605-7422

Conference

Conference	4th Translational Biophotonics: Diagnostics and Therapeutics
Country/Territory	Germany
City	Munich
Period	22-06-25 → 26-06-25

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.3097803

Cite this

Makkithaya, K. N., Chan, M. C., Tseng, W. S., Mazumder, N., & Zhuo, G. Y. (2025). Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis. In Z. Huang, & L. D. Lilge (Eds.), Translational Biophotonics: Diagnostics and Therapeutics IV Article 1393439 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13934). SPIE. https://doi.org/10.1117/12.3097803

Makkithaya, Kausalya Neelavara ; Chan, Ming Che ; Tseng, Wei Shiuan et al. / Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis. Translational Biophotonics: Diagnostics and Therapeutics IV. editor / Zhiwei Huang ; Lothar D. Lilge. SPIE, 2025. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{13365955f2444351a879cdd267f1c5a5,

title = "Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis",

abstract = "We demonstrate a novel approach combining two-photon microscopy with machine learning algorithms to analyze collagen organization in esophageal tissues, enabling differentiation between squamous cell carcinoma and high-grade dysplasia through quantitative extracellular matrix assessment.",

author = "Makkithaya, \{Kausalya Neelavara\} and Chan, \{Ming Che\} and Tseng, \{Wei Shiuan\} and Nirmal Mazumder and Zhuo, \{Guan Yu\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Author(s); 4th Translational Biophotonics: Diagnostics and Therapeutics ; Conference date: 22-06-2025 Through 26-06-2025",

year = "2025",

month = dec,

day = "18",

doi = "10.1117/12.3097803",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Zhiwei Huang and Lilge, \{Lothar D.\}",

booktitle = "Translational Biophotonics",

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Makkithaya, KN, Chan, MC, Tseng, WS, Mazumder, N & Zhuo, GY 2025, Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis. in Z Huang & LD Lilge (eds), Translational Biophotonics: Diagnostics and Therapeutics IV., 1393439, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 13934, SPIE, 4th Translational Biophotonics: Diagnostics and Therapeutics, Munich, Germany, 22-06-25. https://doi.org/10.1117/12.3097803

Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis. / Makkithaya, Kausalya Neelavara; Chan, Ming Che; Tseng, Wei Shiuan et al.
Translational Biophotonics: Diagnostics and Therapeutics IV. ed. / Zhiwei Huang; Lothar D. Lilge. SPIE, 2025. 1393439 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13934).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis

AU - Makkithaya, Kausalya Neelavara

AU - Chan, Ming Che

AU - Tseng, Wei Shiuan

AU - Mazumder, Nirmal

AU - Zhuo, Guan Yu

PY - 2025/12/18

Y1 - 2025/12/18

N2 - We demonstrate a novel approach combining two-photon microscopy with machine learning algorithms to analyze collagen organization in esophageal tissues, enabling differentiation between squamous cell carcinoma and high-grade dysplasia through quantitative extracellular matrix assessment.

AB - We demonstrate a novel approach combining two-photon microscopy with machine learning algorithms to analyze collagen organization in esophageal tissues, enabling differentiation between squamous cell carcinoma and high-grade dysplasia through quantitative extracellular matrix assessment.

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AN - SCOPUS:105025919692

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Translational Biophotonics

A2 - Huang, Zhiwei

A2 - Lilge, Lothar D.

PB - SPIE

T2 - 4th Translational Biophotonics: Diagnostics and Therapeutics

Y2 - 22 June 2025 through 26 June 2025

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Makkithaya KN, Chan MC, Tseng WS, Mazumder N, Zhuo GY. Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis. In Huang Z, Lilge LD, editors, Translational Biophotonics: Diagnostics and Therapeutics IV. SPIE. 2025. 1393439. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.3097803

Machine learning-enhanced two-photon microscopy for the detection of esophageal cancer progression through collagen analysis

Abstract

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All Science Journal Classification (ASJC) codes

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