Abstract
Mangiferin (MGN), a dietary C-glucosylxanthone present in Mangifera indica, is known to possess a spectrum of beneficial pharmacological properties. This study demonstrates antigenotoxic potential of MGN against mercuric chloride (HgCl2) induced genotoxicity in HepG2 cell line. Treatment of HepG2 cells with various concentrations of HgCl2 for 3 h caused a dose-dependent increase in micronuclei frequency and elevation in DNA strand breaks (olive tail moment and tail DNA). Pretreatment with MGN significantly (p < 0.01) inhibited HgCl2-induced (20 μM for 30 h) DNA damage. An optimal antigenotoxic effect of MGN, both in micronuclei and comet assay, was observed at a concentration of 50 μM. Furthermore, HepG2 cells treated with various concentrations of HgCl2 resulted in a dose-dependent increase in the dichlorofluorescein fluorescence, indicating an increase in the generation of reactive oxygen species (ROS). However, MGN by itself failed to generate ROS at a concentration of 50 μM, whereas it could significantly decrease HgCl2-induced ROS. Our study clearly demonstrates that MGN pretreatment reduced the HgCl2-induced DNA damage in HepG2 cells, thus demonstrating the genoprotective potential of MGN, which is mediated mainly by the inhibition of oxidative stress.
| Original language | English |
|---|---|
| Pages (from-to) | 108-116 |
| Number of pages | 9 |
| Journal | Journal of Biochemical and Molecular Toxicology |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 03-2011 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Toxicology
- Health, Toxicology and Mutagenesis
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In: Journal of Biochemical and Molecular Toxicology, Vol. 25, No. 2, 03.2011, p. 108-116.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Mangiferin: A xanthone attenuates mercury chloride induced cytotoxicity and genotoxicity in HepG2 cells
AU - Kaivalya, Mudholkar
AU - Rao, B. Nageshwar
AU - Satish Rao, B. S.
N1 - Cited By :10 Export Date: 11 November 2017 CODEN: JBMTF Correspondence Address: Satish Rao, B.S.; Division of Radiobiology and Toxicology, Manipal Life Sciences Centre, Manipal University, Manipal 576 104, India; email: [email protected] Chemicals/CAS: dichlorofluorescein, 18362-80-6, 2320-96-9, 26836-01-1, 76-54-0, 81-87-8; mangiferin, 4773-96-0; mercury chloride, 51312-24-4; Mercuric Chloride, 7487-94-7; Reactive Oxygen Species; Xanthones; mangiferin, 4773-96-0; xanthone, 90-47-1 Manufacturers: Sigma References: Bridges, C.C., Zalups, R.K., Molecular and ionic mimicry and the transport of toxic metals (2005) Toxicol Appl Pharmacol, 204, pp. 274-308; Baird, C., Cann, M., (2004) Environmental chemistry, , New York: W. H. 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In Toxicology of metals: Biochemical aspects, pp. 373-406. , Berlin: Springer-Verlag; Amorim, M.I., Mergler, D., Bahia, M.O., Dubeau, H., Miranda, D., Lebel, J., Burbano, R.R., Lucotte, M., Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon (2000) An Acad Bras Cienc, 72, pp. 497-507; Betti, C., Davini, T., He, J., Barale, R., Liquid holding effects on methylmercury genotoxicity in human lymphocytes (1993) Mutat Res, 301, pp. 267-273; Fenech, M., Rinaldi, J., Surralles, J., The origin of micronuclei induced by cytosine arabinoside and its synergistic interaction with hydroxyurea in human lymphocytes (1994) Mutagenesis, 9, pp. 273-277; Sobhika, A., Nageshwar Rao, B., Kaivalya, M., Ridhirama, B., Satish Rao, B.S., Mangiferin, a dietary xanthone protects against mercury-induced toxicity in HepG2 cells. Environ Toxicol (DOI 10.1002/tox.20620); Kasi Viswanadh, E., Nageshwar Rao, B., Satish Rao, B.S., Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride (2010) Hum Exp Toxicol, 29, pp. 409-418; Sato, T., Kawamoto, A., Tamura, A., Tatsumi, Y., Fujii, T., Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid) (1992) Chem Pharm Bull (Tokyo), 40, pp. 721-724
PY - 2011/3
Y1 - 2011/3
N2 - Mangiferin (MGN), a dietary C-glucosylxanthone present in Mangifera indica, is known to possess a spectrum of beneficial pharmacological properties. This study demonstrates antigenotoxic potential of MGN against mercuric chloride (HgCl2) induced genotoxicity in HepG2 cell line. Treatment of HepG2 cells with various concentrations of HgCl2 for 3 h caused a dose-dependent increase in micronuclei frequency and elevation in DNA strand breaks (olive tail moment and tail DNA). Pretreatment with MGN significantly (p < 0.01) inhibited HgCl2-induced (20 μM for 30 h) DNA damage. An optimal antigenotoxic effect of MGN, both in micronuclei and comet assay, was observed at a concentration of 50 μM. Furthermore, HepG2 cells treated with various concentrations of HgCl2 resulted in a dose-dependent increase in the dichlorofluorescein fluorescence, indicating an increase in the generation of reactive oxygen species (ROS). However, MGN by itself failed to generate ROS at a concentration of 50 μM, whereas it could significantly decrease HgCl2-induced ROS. Our study clearly demonstrates that MGN pretreatment reduced the HgCl2-induced DNA damage in HepG2 cells, thus demonstrating the genoprotective potential of MGN, which is mediated mainly by the inhibition of oxidative stress.
AB - Mangiferin (MGN), a dietary C-glucosylxanthone present in Mangifera indica, is known to possess a spectrum of beneficial pharmacological properties. This study demonstrates antigenotoxic potential of MGN against mercuric chloride (HgCl2) induced genotoxicity in HepG2 cell line. Treatment of HepG2 cells with various concentrations of HgCl2 for 3 h caused a dose-dependent increase in micronuclei frequency and elevation in DNA strand breaks (olive tail moment and tail DNA). Pretreatment with MGN significantly (p < 0.01) inhibited HgCl2-induced (20 μM for 30 h) DNA damage. An optimal antigenotoxic effect of MGN, both in micronuclei and comet assay, was observed at a concentration of 50 μM. Furthermore, HepG2 cells treated with various concentrations of HgCl2 resulted in a dose-dependent increase in the dichlorofluorescein fluorescence, indicating an increase in the generation of reactive oxygen species (ROS). However, MGN by itself failed to generate ROS at a concentration of 50 μM, whereas it could significantly decrease HgCl2-induced ROS. Our study clearly demonstrates that MGN pretreatment reduced the HgCl2-induced DNA damage in HepG2 cells, thus demonstrating the genoprotective potential of MGN, which is mediated mainly by the inhibition of oxidative stress.
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U2 - 10.1002/jbt.20366
DO - 10.1002/jbt.20366
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C2 - 21308892
AN - SCOPUS:79953709130
SN - 1095-6670
VL - 25
SP - 108
EP - 116
JO - Journal of Biochemical and Molecular Toxicology
JF - Journal of Biochemical and Molecular Toxicology
IS - 2
ER -