Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells

Aseefhali Bankapur; R. Sagar Krishnamurthy; Elsa Zachariah; Chidangil Santhosh; Basavaraj Chougule; Bhavishna Praveen; Manna Valiathan; Deepak Mathur

doi:10.1371/journal.pone.0035075

Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells

Aseefhali Bankapur, R. Sagar Krishnamurthy, Elsa Zachariah, Chidangil Santhosh, Basavaraj Chougule, Bhavishna Praveen, Manna Valiathan, Deepak Mathur

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 μg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 μg/ml.

Original language	English
Article number	e35075
Journal	PLoS One
Volume	7
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0035075
Publication status	Published - 13-04-2012

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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abstract = "We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 μg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 μg/ml.",

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AU - Bankapur, Aseefhali

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AU - Chougule, Basavaraj

AU - Praveen, Bhavishna

AU - Valiathan, Manna

AU - Mathur, Deepak

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Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells

Abstract

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