TY - JOUR
T1 - Micro-Raman spectroscopy study of blood samples from myocardial infarction patients
AU - John, Reena V.
AU - Devasia, Tom
AU - N, Mithun
AU - Lukose, Jijo
AU - Chidangil, Santhosh
N1 - Funding Information:
Open access funding provided by Manipal Academy of Higher Education, Manipal The authors are thankful to DBT, Govt. of India and VGST (GRD No. 459) Govt. of Karnataka, for the micro-Raman facility and financial support to establish the biophotonics laboratory under the scheme of Centre of Excellence in Science, Engineering and Medicine (CESEM) respectively. Ms. Reena V John is thankful to the Directorate of Minorities, Govt. of Karnataka, for the fellowship and Manipal Academy of Higher Education, Manipal, for providing other laboratory facilities.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high potential in probing biochemical changes in clinical samples during initiation and progress of diseases. In this work, blood was taken as the sample to examine inflammation in acute MI patients using Raman spectroscopy. Ratio of Raman peak intensities that corresponds to phenylalanine (1000 cm−1) and tyrosine (825 cm−1) can facilitate indirect information about tetrahydrobiopterin (BH4) availability, which can indicate inflammatory status in patients. This ratio obtained was higher for MI patients in comparison with control subjects. The decrease in phenylalanine and tyrosine ratio (Phe-Tyr ratio) is attributed to the prognosis of standard of care (medications like antiplatelets including aspirin, statin and revascularisation) leading to inflammation reduction. Phe-Tyr ratio estimated from the Raman spectra of blood can be exploited as a reliable method to probe inflammation due to MI. The method is highly objective, require only microliters of sample and minimal sample preparation, signifying its clinical utility.
AB - Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high potential in probing biochemical changes in clinical samples during initiation and progress of diseases. In this work, blood was taken as the sample to examine inflammation in acute MI patients using Raman spectroscopy. Ratio of Raman peak intensities that corresponds to phenylalanine (1000 cm−1) and tyrosine (825 cm−1) can facilitate indirect information about tetrahydrobiopterin (BH4) availability, which can indicate inflammatory status in patients. This ratio obtained was higher for MI patients in comparison with control subjects. The decrease in phenylalanine and tyrosine ratio (Phe-Tyr ratio) is attributed to the prognosis of standard of care (medications like antiplatelets including aspirin, statin and revascularisation) leading to inflammation reduction. Phe-Tyr ratio estimated from the Raman spectra of blood can be exploited as a reliable method to probe inflammation due to MI. The method is highly objective, require only microliters of sample and minimal sample preparation, signifying its clinical utility.
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U2 - 10.1007/s10103-022-03604-1
DO - 10.1007/s10103-022-03604-1
M3 - Article
C2 - 35821543
AN - SCOPUS:85134260695
SN - 0268-8921
VL - 37
SP - 3451
EP - 3460
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 9
ER -