TY - JOUR
T1 - Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa
AU - Suresh Poojary, Pooja
AU - Shreya, Ajjappla Basavaraj
AU - Mutalik, Srinivas
AU - Sachdev, Divya
AU - Taneja, Neetu K.
AU - Raghu, Shamprasad Varija
AU - Pasricha, Renu
AU - Ananda, Hanumappa
AU - Pramoda, K.
AU - Vani Lakshmi, R.
AU - Adiga, Satish Kumar
AU - Kalthur, Guruprasad
N1 - Publisher Copyright:
© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2024
Y1 - 2024
N2 - Zinc, an essential micronutrient, is an important mediator of sperm function. Supplementation of zinc oxide nanoparticle (ZnONPs) to semen cryopreservation medium has shown beneficial effects on sperm function, mainly due to its antioxidant and membrane stabilization properties. However, the ZnONPs are known to be membrane impermeable. In the current study, we explored the beneficial properties of membrane permeable zinc oxide nanoparticles on semen cryopreservation outcome. For the study, 52 liquefied human ejaculates were cryopreserved with chitosan-coated ZnONPs and stored in liquid nitrogen for one week. Samples were thawed and assessed for the sperm functional characters and DNA integrity. Significantly higher motility (P < 0.05), prolonged survival (P < 0.05) and lower DNA damage (P < 0.05) was observed in spermatozoa of semen samples cryopreserved with chitosan-coated ZnONPs. In conclusion, the presence of chitosan-coated ZnONPs in the ejaculate prior to cryopreservation increases the survival and decreases DNA damage without compromising with the functional competence of spermatozoa.
AB - Zinc, an essential micronutrient, is an important mediator of sperm function. Supplementation of zinc oxide nanoparticle (ZnONPs) to semen cryopreservation medium has shown beneficial effects on sperm function, mainly due to its antioxidant and membrane stabilization properties. However, the ZnONPs are known to be membrane impermeable. In the current study, we explored the beneficial properties of membrane permeable zinc oxide nanoparticles on semen cryopreservation outcome. For the study, 52 liquefied human ejaculates were cryopreserved with chitosan-coated ZnONPs and stored in liquid nitrogen for one week. Samples were thawed and assessed for the sperm functional characters and DNA integrity. Significantly higher motility (P < 0.05), prolonged survival (P < 0.05) and lower DNA damage (P < 0.05) was observed in spermatozoa of semen samples cryopreserved with chitosan-coated ZnONPs. In conclusion, the presence of chitosan-coated ZnONPs in the ejaculate prior to cryopreservation increases the survival and decreases DNA damage without compromising with the functional competence of spermatozoa.
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U2 - 10.1080/16583655.2024.2422648
DO - 10.1080/16583655.2024.2422648
M3 - Article
AN - SCOPUS:85209785423
SN - 1658-3655
VL - 18
JO - Journal of Taibah University for Science
JF - Journal of Taibah University for Science
IS - 1
M1 - 2422648
ER -