TY - JOUR
T1 - Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST)
T2 - A study from tertiary care center in India
AU - Chaudhry, Rama
AU - Sharma, Nidhi
AU - Bahadur, Tej
AU - Khullar, Swati
AU - Agarwal, Sonu Kumari
AU - Gahlowt, Abhipray
AU - Gupta, Nitin
AU - Kumar, Lalit
AU - Kabra, Sushil Kumar
AU - Dey, Aparajit B.
N1 - Publisher Copyright:
© 2022
PY - 2022/6
Y1 - 2022/6
N2 - Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.
AB - Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.
UR - https://www.scopus.com/pages/publications/85127305647
UR - https://www.scopus.com/pages/publications/85127305647#tab=citedBy
U2 - 10.1016/j.anaerobe.2022.102545
DO - 10.1016/j.anaerobe.2022.102545
M3 - Article
C2 - 35278711
AN - SCOPUS:85127305647
SN - 1075-9964
VL - 75
JO - Anaerobe
JF - Anaerobe
M1 - 102545
ER -
