Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India

Rama Chaudhry; Nidhi Sharma; Tej Bahadur; Swati Khullar; Sonu Kumari Agarwal; Abhipray Gahlowt; Nitin Gupta; Lalit Kumar; Sushil Kumar Kabra; Aparajit B. Dey

doi:10.1016/j.anaerobe.2022.102545

Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India

Rama Chaudhry^*
, Nidhi Sharma
, Tej Bahadur
, Swati Khullar
, Sonu Kumari Agarwal
, Abhipray Gahlowt
, Nitin Gupta
, Lalit Kumar
, Sushil Kumar Kabra
, Aparajit B. Dey

^*Corresponding author for this work

Department of Infectious Diseases, Kasturba Medical College, Manipal

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.

Original language	English
Article number	102545
Journal	Anaerobe
Volume	75
DOIs	https://doi.org/10.1016/j.anaerobe.2022.102545
Publication status	Published - 06-2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Microbiology
Infectious Diseases

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10.1016/j.anaerobe.2022.102545

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@article{43daa6f090f14a0297c28c17a6f113f2,

title = "Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India",

abstract = "Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.",

author = "Rama Chaudhry and Nidhi Sharma and Tej Bahadur and Swati Khullar and Agarwal, \{Sonu Kumari\} and Abhipray Gahlowt and Nitin Gupta and Lalit Kumar and Kabra, \{Sushil Kumar\} and Dey, \{Aparajit B.\}",

note = "Publisher Copyright: {\textcopyright} 2022",

year = "2022",

month = jun,

doi = "10.1016/j.anaerobe.2022.102545",

language = "English",

volume = "75",

journal = "Anaerobe",

issn = "1075-9964",

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TY - JOUR

T1 - Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST)

T2 - A study from tertiary care center in India

AU - Chaudhry, Rama

AU - Sharma, Nidhi

AU - Bahadur, Tej

AU - Khullar, Swati

AU - Agarwal, Sonu Kumari

AU - Gahlowt, Abhipray

AU - Gupta, Nitin

AU - Kumar, Lalit

AU - Kabra, Sushil Kumar

AU - Dey, Aparajit B.

PY - 2022/6

Y1 - 2022/6

N2 - Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.

AB - Objectives: Clostridioides difficile is an important pathogen responsible for antibiotic-associated diarrhoea (AAD). This study was aimed to perform multi-locus sequence typing (MLST) of C. difficile isolates from AAD cases and to understand the clonal relationship between these C. difficile strains. Methods: Thirty five strains and a standard strain C.difficile ATCC 9689 were characterized by polymerase chain reaction assay (PCR) for toxin genes (tcdA and tcdB gene) detection and MLST. Results: MLST results revealed that the most common sequence types were ST-17, ST-54, ST-63. The cluster analysis revealed that strains isolated from AAD patients generated 12 MLST sequence types grouped into two distinct evolutionary lineages. Conclusions: ST 17 is most prominent sequence type. This is the first report of MLST based study of C. difficile from India. Further studies from diverse geographical regions can help better understand the epidemiology of CDI in India.

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DO - 10.1016/j.anaerobe.2022.102545

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AN - SCOPUS:85127305647

SN - 1075-9964

VL - 75

JO - Anaerobe

JF - Anaerobe

M1 - 102545

ER -

Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India

Abstract

UN SDGs

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