TY - JOUR
T1 - Mueller matrix signature in advanced fluorescence microscopy imaging
AU - Mazumder, Nirmal
AU - Qiu, Jianjun
AU - Kao, Fu Jen
AU - Diaspro, Alberto
N1 - Publisher Copyright:
© 2017 IOP Publishing Ltd.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.
AB - We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.
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U2 - 10.1088/2040-8986/aa5114
DO - 10.1088/2040-8986/aa5114
M3 - Article
AN - SCOPUS:85011416650
SN - 2040-8978
VL - 19
JO - Journal of Optics (United Kingdom)
JF - Journal of Optics (United Kingdom)
IS - 2
M1 - 025301
ER -