Multidimensional HPTLC: Instrumentation, Techniques, and Applications

High-performance thin-layer chromatography (HPTLC) is a modern analytical separation technique that shares its basic principles with traditional thin-layer chromatography (TLC). HPTLC relies on the separation of chemical compounds based on their differential migration rates on a thin layer of adsorbent material (usually silica gel or cellulose) coated on a glass or aluminum plate. However, HPTLC builds upon these principles with several enhancements for improved performance and precision. HPTLC has a wide range of applications in various fields, including pharmaceuticals, food and beverage analysis, environmental monitoring, and forensic science. HPTLC’s efficiency, speed, and versatility make it a valuable tool in laboratories and industries for qualitative and quantitative analysis. Its adaptability to various sample types and its cost-effectiveness makes it a preferred choice for many analytical challenges. As technology continues to advance, HPTLC will likely remain an essential technique in the field of analytical chemistry.