TY - JOUR
T1 - Multifunctional and multilayer surgical sealant for a better patient safety
AU - Chevala, Naga Thirumalesh
AU - Kumar, Lalit
AU - Veetilvalappil, Vimal
AU - Mathew, Aranjani Jesil
AU - Jadhav, Manali
AU - Gandhi, Mayuri
AU - Rao, C. Mallikarjuna
N1 - Funding Information:
All authors would like to thank Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal (India), for providing all the required facilities to carry out the research smoothly. The whole research team thanks the Indian Council of Medical Research, Delhi, for providing the ICMR-SRF grant as a fellowship to Naga Thirumalesh Chevala (ICMR-SRF, grant number: 45/07/2020-Hae/BMS). And we would like to thank the Centre for Research in Nanotechnology and Science (CRNTS), Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology, Bombay, for providing an FTIR chemical imaging facility. Naga Thirumalesh Chevala: Conceptualization, Data curation, Formal analysis, Methodology, Writing-original draft; Lalit Kumar: Resources, Investigation, Supervision, Visualization and Methodology, Formal analysis, and Writing-review & editing; C Mallikarjuna Rao: Supervision, and Visualization; Vimal, Aranjani Jesil Mathew,Mayuri Gandhi and Manali Jadhav: Data curation, Methodology and Formal analysis.
Funding Information:
All authors would like to thank Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal (India), for providing all the required facilities to carry out the research smoothly. The whole research team thanks the Indian Council of Medical Research, Delhi , for providing the ICMR-SRF grant as a fellowship to Naga Thirumalesh Chevala (ICMR-SRF, grant number: 45/07/2020-Hae/BMS ). And we would like to thank the Centre for Research in Nanotechnology and Science (CRNTS), Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology, Bombay, for providing an FTIR chemical imaging facility.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/12/15
Y1 - 2022/12/15
N2 - Unmanagable bleeding during combats, road accidents, and intraoperative or external injuries causes a significant rise in mortality. Any biomaterial that can intensify hemostasis, and reduce complications, can reduce mortality and increase the survivability of the subjects. In the present research, we attempted to develop a multifunctional surgical sealant (MfSS) by integrating fast disintegrating film (FDF), nanoporous fibers reinforced composite scaffold (NFRCS), and a flexible silicon layer (FSL). By integrating FDF, NFRCS, and FSL, MfSS was developed. MfSS comprises four layers: two FDFs, one NFRCS, and one FSL. The FSL was surface coated with tissue adhesive glue that retains the MfSS at the application and controls the pressure excited by the blood. The multi-functionality of the MfSS was attained by loading tranexamic acid (TXA) and Epigallocatechin gallate (EGCG) in FDF. The developed FDFs rapidly disintegrate at the application site in the blood pool, help attain high drug concentrations at the application site, and prevent drug washout because of blood. The in vitro characterization studies confirm the possibility of developing the MfSS with four different layers and FDF disintegration in citrated rat blood. The in vivo BCT assay confirms the MfSS activates and intensifies the blood coagulation process in two animal models. The MfSS could regulate the microenvironment, and TXA and EGCG loaded in the FDF could act at the cellular level, resulting in better wound healing in the excision wound model.
AB - Unmanagable bleeding during combats, road accidents, and intraoperative or external injuries causes a significant rise in mortality. Any biomaterial that can intensify hemostasis, and reduce complications, can reduce mortality and increase the survivability of the subjects. In the present research, we attempted to develop a multifunctional surgical sealant (MfSS) by integrating fast disintegrating film (FDF), nanoporous fibers reinforced composite scaffold (NFRCS), and a flexible silicon layer (FSL). By integrating FDF, NFRCS, and FSL, MfSS was developed. MfSS comprises four layers: two FDFs, one NFRCS, and one FSL. The FSL was surface coated with tissue adhesive glue that retains the MfSS at the application and controls the pressure excited by the blood. The multi-functionality of the MfSS was attained by loading tranexamic acid (TXA) and Epigallocatechin gallate (EGCG) in FDF. The developed FDFs rapidly disintegrate at the application site in the blood pool, help attain high drug concentrations at the application site, and prevent drug washout because of blood. The in vitro characterization studies confirm the possibility of developing the MfSS with four different layers and FDF disintegration in citrated rat blood. The in vivo BCT assay confirms the MfSS activates and intensifies the blood coagulation process in two animal models. The MfSS could regulate the microenvironment, and TXA and EGCG loaded in the FDF could act at the cellular level, resulting in better wound healing in the excision wound model.
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UR - http://www.scopus.com/inward/citedby.url?scp=85143180548&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2022.122411
DO - 10.1016/j.ijpharm.2022.122411
M3 - Article
C2 - 36402288
AN - SCOPUS:85143180548
SN - 0378-5173
VL - 629
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 122411
ER -