Mutant MESD links cellular stress to type I collagen aggregation in osteogenesis imperfecta type XX

Debasish Kumar Ghosh, Prajna Udupa, Akshaykumar Nanaji Shrikondawar, Gandham Sri Lakshmi Bhavani, Hitesh Shah, Akash Ranjan, Katta M. Girisha

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Aberrant forms of endoplasmic reticulum (ER)-resident chaperones are implicated in loss of protein quality control in rare diseases. Here we report a novel mutation (p.Asp233Asn) in the ER retention signal of MESD by whole exome sequencing of an individual diagnosed with osteogenesis imperfecta (OI) type XX. While MESDD233N has similar stability and chaperone activity as wild-type MESD, its mislocalization to cytoplasm leads to imbalance of ER proteostasis, resulting in improper folding and aggregation of proteins, including LRP5 and type I collagen. Aggregated LRP5 loses its plasma membrane localization to disrupt the expression of WNT-responsive genes, such as BMP2, BMP4, in proband fibroblasts. We show that MESD is a direct chaperone of pro-α1(I) [COL1A1], and absence of MESDD233N in ER results in cytosolic type I collagen aggregates that remain mostly not secreted. While cytosolic type I collagen aggregates block the intercellular nanotubes, decreased extracellular type I collagen also results in loss of interaction of ITGB1 with type I collagen and weaker attachment of fibroblasts to matrix. Although proband fibroblasts show increased autophagy to degrade the aggregated type I collagen, an overall cellular stress overwhelms the proband fibroblasts. In summary, we present an essential chaperone function of MESD for LRP5 and type I collagen and demonstrating how the D233N mutation in MESD correlates with impaired WNT signaling and proteostasis in OI.

Original languageEnglish
Pages (from-to)81-106
Number of pages26
JournalMatrix Biology
Publication statusPublished - 01-2023

All Science Journal Classification (ASJC) codes

  • Molecular Biology


Dive into the research topics of 'Mutant MESD links cellular stress to type I collagen aggregation in osteogenesis imperfecta type XX'. Together they form a unique fingerprint.

Cite this