TY - JOUR
T1 - NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques
T2 - A new biomarker for assessment of embryo implantation potential
AU - Pudakalakatti, Shivanand M.
AU - Uppangala, Shubhashree
AU - D'Souza, Fiona
AU - Kalthur, Guruprasad
AU - Kumar, Pratap
AU - Adiga, Satish Kumar
AU - Atreya, Hanudatta S.
PY - 2013/1
Y1 - 2013/1
N2 - There has been growing interest in understanding energy metabolism in human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of the method. Using NMR spectroscopy as a noninvasive tool, we studied human embryo metabolism to identify specific biomarkers to assess the quality of embryos for their implantation potential. The study was based on estimation of pyruvate, lactate and alanine levels in the growth medium, ISM1, used in the culture of embryos. An NMR study involving 127 embryos from 48 couples revealed that embryos transferred on Day 3 (after 72h in vitro culture) with successful implantation (pregnancy) exhibited significantly (p<10-5) lower pyruvate/alanine ratios compared to those that failed to implant. Lactate levels in media were similar for all embryos. This implies that in addition to lactate production, successfully implanted embryos use pyruvate to produce alanine and other cellular functions. While pyruvate and alanine individually have been used as biomarkers, the present study highlights the potential of combining them to provide a single parameter that correlates strongly with implantation potential.
AB - There has been growing interest in understanding energy metabolism in human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of the method. Using NMR spectroscopy as a noninvasive tool, we studied human embryo metabolism to identify specific biomarkers to assess the quality of embryos for their implantation potential. The study was based on estimation of pyruvate, lactate and alanine levels in the growth medium, ISM1, used in the culture of embryos. An NMR study involving 127 embryos from 48 couples revealed that embryos transferred on Day 3 (after 72h in vitro culture) with successful implantation (pregnancy) exhibited significantly (p<10-5) lower pyruvate/alanine ratios compared to those that failed to implant. Lactate levels in media were similar for all embryos. This implies that in addition to lactate production, successfully implanted embryos use pyruvate to produce alanine and other cellular functions. While pyruvate and alanine individually have been used as biomarkers, the present study highlights the potential of combining them to provide a single parameter that correlates strongly with implantation potential.
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U2 - 10.1002/nbm.2814
DO - 10.1002/nbm.2814
M3 - Article
C2 - 22714820
AN - SCOPUS:84871690655
SN - 0952-3480
VL - 26
SP - 20
EP - 27
JO - NMR in Biomedicine
JF - NMR in Biomedicine
IS - 1
ER -