Nuclear DNA fragmentation negatively affects zona binding competence of y bearing mouse spermatozoa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Purpose: To investigate the influence of sperm DNA integrity on the zona binding ability of mouse spermatozoa in relation to their sex chromosomal constitution. Method(s): In this prospective experimental study, the sperm DNA fragmentation was induced by exposing testicular area of Swiss Albino mice (Mus musculus) to different doses of γ-radiation (0, 2.5, 5.0 and 10.0 Gy). Sperm DNA fragmentation was quantified by single cell gel electrophoresis (comet assay). In vitro sperm zona binding assay was performed and the numbers of zona bound X and Y bearing spermatozoa were determined using fluorescence in situ hybridization (FISH). Result(s): The assessment of zona pellucida bound X and Y-bearing spermatozoa using fluorescence in situ hybridization has revealed a unique binding pattern. The number of zona bound Y-spermatozoa declined significantly (P < 0.01 to 0.0001) with increase in the DNA damage. The skewed binding pattern of X and Y-bearing sperm was strongly correlated with the extent of sperm DNA damage. Conclusion(s): The zona pellucida may have a role in preventing DNA damaged mouse sperm binding especially towards Y-bearing sperm. However, the exact mechanism behind this observation needs to be elucidated further.

Original languageEnglish
Pages (from-to)1611-1615
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Issue number12
Publication statusPublished - 12-2013

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)


Dive into the research topics of 'Nuclear DNA fragmentation negatively affects zona binding competence of y bearing mouse spermatozoa'. Together they form a unique fingerprint.

Cite this