Optical Trap Combined with Raman Spectroscopy to Probe Red Blood Cell Behaviour in Certain Intravenous Fluids

Jijo Lukose, Ganesh Mohan, N. Mithun, Shamee Shastry, C. Santhosh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Systematic studies on a live, individual human red blood cell without any chemical fixation can be realized with the aid of optical tweezers. In the present work, the behavior of human red blood cell in dextrose containing intravenous fluids and blood plasma were investigated and compared using a home-made near-IR Laser Tweezers Raman spectrometer. Significant spectral variations in Raman signatures exist between the RBCs in plasma and dextrose containing intravenous fluids, which mainly comprise of the hemoglobin oxygenation-deoxygenation transition as well as the heme aggregation markers.

Original languageEnglish
Article number040302
JournalJournal of Biomedical Photonics and Engineering
Volume5
Issue number4
DOIs
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Acoustics and Ultrasonics

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