TY - JOUR
T1 - Oral candida carriage and morphotype differentiation in chronic periodontitis patients with and without diabetes in the Indian sub-continent
AU - Venkatesan, Gomathinayagam
AU - Uppoor, Ashita
AU - Naik, Dilip
AU - Kadkampally, David
AU - Maddi, Abhiram
N1 - Funding Information:
We would like to thank Frank Scannapieco, Mine Tezal and Amarpreet Sabharwal, School of Dental Medicine, State University of New York at Buffalo, for reviewing our manuscript and providing critical suggestions. This study was funded intramurally by Department of Periodontology, Manipal College of Dental Sciences, Manipal University, Mangalore, Karnataka, India.
Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2015/12
Y1 - 2015/12
N2 - The aim of this study was to assess the oral Candida carriage and morphotype differentiation of Candida species in chronic periodontitis patients, with and without diabetes mellitus. This cross sectional study included 30 subjects in the age range of 40-60 years, who were divided into two groups: 15 chronic periodontitis only (CP) patients, and 15 chronic periodontitis patients with diabetes (CPD). Clinical measurements included plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL), and fasting blood sugar level (FBS). The unstimulated whole saliva samples were collected for fungal analysis. Candida carriage was analyzed by measuring colony forming units (CFU) following the culture of samples. Qualitative morphotype differentiation of Candida species from yeast to hyphal form was analyzed using Periodic acid-Schiff (PAS) staining. There was no statistically significant difference between CP and CPD groups for the periodontal parameters. However, a significantly higher Candida species CFU count was found in CPD (0.33 ± 0.23) as compared to CP (0.05 ± 0.04) group. This pilot study suggests that the occurrence of Candida species is higher in the saliva of chronic periodontitis patients with diabetes as compared to patients with chronic periodontitis alone.
AB - The aim of this study was to assess the oral Candida carriage and morphotype differentiation of Candida species in chronic periodontitis patients, with and without diabetes mellitus. This cross sectional study included 30 subjects in the age range of 40-60 years, who were divided into two groups: 15 chronic periodontitis only (CP) patients, and 15 chronic periodontitis patients with diabetes (CPD). Clinical measurements included plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL), and fasting blood sugar level (FBS). The unstimulated whole saliva samples were collected for fungal analysis. Candida carriage was analyzed by measuring colony forming units (CFU) following the culture of samples. Qualitative morphotype differentiation of Candida species from yeast to hyphal form was analyzed using Periodic acid-Schiff (PAS) staining. There was no statistically significant difference between CP and CPD groups for the periodontal parameters. However, a significantly higher Candida species CFU count was found in CPD (0.33 ± 0.23) as compared to CP (0.05 ± 0.04) group. This pilot study suggests that the occurrence of Candida species is higher in the saliva of chronic periodontitis patients with diabetes as compared to patients with chronic periodontitis alone.
UR - http://www.scopus.com/inward/record.url?scp=85069496431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069496431&partnerID=8YFLogxK
U2 - 10.3390/dj3040123
DO - 10.3390/dj3040123
M3 - Article
AN - SCOPUS:85069496431
SN - 2304-6767
VL - 3
SP - 123
EP - 131
JO - Dentistry Journal
JF - Dentistry Journal
IS - 4
ER -
