TY - JOUR
T1 - Pelvic irradiation induces behavioural and neuronal damage through gut dysbiosis in a rat model
AU - Venkidesh, Babu Santhi
AU - Narasimhamurthy, Rekha K.
AU - Jnana, Apoorva
AU - Reghunathan, Dinesh
AU - Sharan, Krishna
AU - Chandraguthi, Srinidhi G.
AU - Saigal, Mehreen
AU - Murali, Thokur S.
AU - Mumbrekar, Kamalesh Dattaram
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Radiation exposure can cause gut dysbiosis and there is a positive correlation between gut microbial imbalance and radiation-induced side effects in cancer patients. However, the influence of radiation on the gut-brain axis (GBA) and its neurological consequences are not well understood. Therefore, this study aimed to investigate the impact of pelvic irradiation on gut microbiota and the brain. Sprague Dawley rats were irradiated with a single dose of 6 Gy, and faecal samples were collected at different time points (7 and 12-days post-irradiation) for microbial analysis. Behavioural, histological, and gene expression analysis were performed to assess the effect of microbial dysbiosis on the brain. The findings indicated alterations in microbial diversity, disrupted intestinal morphology and integrity, neuronal death-related brain changes, neuroinflammation and reduced locomotor activity. Hippocampal gene expression analysis also showed a reduced expression of neural plasticity-related genes. Overall, this study demonstrated that pelvic irradiation affects gut microbiota, intestinal morphology, integrity, brain neuronal maturation, neural plasticity gene expression, and behaviour.
AB - Radiation exposure can cause gut dysbiosis and there is a positive correlation between gut microbial imbalance and radiation-induced side effects in cancer patients. However, the influence of radiation on the gut-brain axis (GBA) and its neurological consequences are not well understood. Therefore, this study aimed to investigate the impact of pelvic irradiation on gut microbiota and the brain. Sprague Dawley rats were irradiated with a single dose of 6 Gy, and faecal samples were collected at different time points (7 and 12-days post-irradiation) for microbial analysis. Behavioural, histological, and gene expression analysis were performed to assess the effect of microbial dysbiosis on the brain. The findings indicated alterations in microbial diversity, disrupted intestinal morphology and integrity, neuronal death-related brain changes, neuroinflammation and reduced locomotor activity. Hippocampal gene expression analysis also showed a reduced expression of neural plasticity-related genes. Overall, this study demonstrated that pelvic irradiation affects gut microbiota, intestinal morphology, integrity, brain neuronal maturation, neural plasticity gene expression, and behaviour.
UR - https://www.scopus.com/pages/publications/85175332440
UR - https://www.scopus.com/inward/citedby.url?scp=85175332440&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2023.110775
DO - 10.1016/j.cbi.2023.110775
M3 - Article
C2 - 37866488
AN - SCOPUS:85175332440
SN - 0009-2797
VL - 386
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
M1 - 110775
ER -
