TY - JOUR
T1 - Pharmacological approach to increasing the retention of radiation-induced γ-H2AX foci using phosphatase inhibitors
T2 - Significance in radiation biodosimetry
AU - Nayak, Akshaykumar A.
AU - Mumbrekar, Kamalesh Dattaram
AU - Rao, B. S.Satish
PY - 2018/3/1
Y1 - 2018/3/1
N2 - In a scenario of accidental mass radiation exposure transportation and analysis of samples may take some time, resulting in loss of biomarker information over this period. The present study aims to use phosphatase inhibitors for longer retention of focal signals to adopt γ-H2AX as a biodosimetric biomarker for the management of early triage. Peripheral blood lymphocytes isolated from healthy individuals were irradiated in vitro with x-rays and γ-H2AX foci were analysed using fluorescent microscopy and flow cytometric methods. Further, the effect of protein phosphatase 2A inhibitors such as calyculin A, fostriecin and okadiac acid on the retention of foci was studied. Fluorescent microscopy was found to be a more sensitive method than flow cytometry. Calyculin A showed significant retention of focal signals at 6 h with 1.5-fold increased retention compared to radiation alone; this may prove beneficial in early triage management because of a better dose approximation.
AB - In a scenario of accidental mass radiation exposure transportation and analysis of samples may take some time, resulting in loss of biomarker information over this period. The present study aims to use phosphatase inhibitors for longer retention of focal signals to adopt γ-H2AX as a biodosimetric biomarker for the management of early triage. Peripheral blood lymphocytes isolated from healthy individuals were irradiated in vitro with x-rays and γ-H2AX foci were analysed using fluorescent microscopy and flow cytometric methods. Further, the effect of protein phosphatase 2A inhibitors such as calyculin A, fostriecin and okadiac acid on the retention of foci was studied. Fluorescent microscopy was found to be a more sensitive method than flow cytometry. Calyculin A showed significant retention of focal signals at 6 h with 1.5-fold increased retention compared to radiation alone; this may prove beneficial in early triage management because of a better dose approximation.
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U2 - 10.1088/1361-6498/aaa97a
DO - 10.1088/1361-6498/aaa97a
M3 - Article
AN - SCOPUS:85046677054
SN - 0952-4746
VL - 38
SP - 318
EP - 328
JO - Journal of the Society for Radiological Protection
JF - Journal of the Society for Radiological Protection
IS - 1
ER -