TY - JOUR
T1 - Phosphorylation Enhanced the anti-cancer Therapeutic Activity of Larix sibirica-derived Arabinogalactan in Hepatocellular Carcinoma
AU - Shetty, Maya P.
AU - Tambe, Prasad
AU - Rana, Komal
AU - Kulkarni, Suresh D.
AU - Chaudhari, Pradip
AU - Bharati, Sanjay
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - Cancer is one of the major health concern, underscoring the need for the development of potent anticancer therapeutic agents to overcome the challenges associated with conventional drugs. Several polysaccharides inherently possess mild to strong anticancer properties which can be further enhanced by trivial chemical modifications such as phosphorylation. In the present study, we report the anticancer therapeutic properties of phosphorylated arabinogalactan (P-AG). The synthesized compound P-AG was analyzed for its chemical characteristics via FT-IR, NMR and mass spectroscopy. The compound was evaluated for its anti-cancer therapeutic activity in Huh-7 cells followed by in vivo anticancer therapeutic assessment in a diethylnitrosamine-induced hepatocellular carcinoma Wistar rat model. The chemical characterization of P-AG revealed successful phosphorylation of arabinogalactan. P-AG treatment significantly inhibited the growth of Huh-7 cells (IC50 of 250 µg/mL), whereas AG had no effect at a similar dose. P-AG significantly inhibited tumor cell proliferation, impeded angiogenesis and promoted tumor cell apoptosis thereby reducing total number of hepatic tumors. These findings underscore the enhanced anticancer therapeutic potential of P-AG as compared to AG for the treatment of HCC.
AB - Cancer is one of the major health concern, underscoring the need for the development of potent anticancer therapeutic agents to overcome the challenges associated with conventional drugs. Several polysaccharides inherently possess mild to strong anticancer properties which can be further enhanced by trivial chemical modifications such as phosphorylation. In the present study, we report the anticancer therapeutic properties of phosphorylated arabinogalactan (P-AG). The synthesized compound P-AG was analyzed for its chemical characteristics via FT-IR, NMR and mass spectroscopy. The compound was evaluated for its anti-cancer therapeutic activity in Huh-7 cells followed by in vivo anticancer therapeutic assessment in a diethylnitrosamine-induced hepatocellular carcinoma Wistar rat model. The chemical characterization of P-AG revealed successful phosphorylation of arabinogalactan. P-AG treatment significantly inhibited the growth of Huh-7 cells (IC50 of 250 µg/mL), whereas AG had no effect at a similar dose. P-AG significantly inhibited tumor cell proliferation, impeded angiogenesis and promoted tumor cell apoptosis thereby reducing total number of hepatic tumors. These findings underscore the enhanced anticancer therapeutic potential of P-AG as compared to AG for the treatment of HCC.
UR - https://www.scopus.com/pages/publications/105014894986
UR - https://www.scopus.com/pages/publications/105014894986#tab=citedBy
U2 - 10.1007/s12013-025-01876-2
DO - 10.1007/s12013-025-01876-2
M3 - Article
AN - SCOPUS:105014894986
SN - 1085-9195
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
ER -
