Photo-bio modulatory response of platelets to low power laser - A pilot study

Bharath Rathnakar; Namita Bhyravbhatla; Subhash Chandra; Bola Sadashiva Satish Rao; Krishna Kishore Mahato

doi:10.1364/FIO.2017.JTu2A.61

Photo-bio modulatory response of platelets to low power laser - A pilot study

Bharath Rathnakar, Namita Bhyravbhatla, Subhash Chandra, Bola Sadashiva Satish Rao, Krishna Kishore Mahato^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Platelets initiate as well as regulate the cascade of events that take place during tissue repair through haemostatic regulation and secretion of growth factors and cytokines. In the present study the effect of 830 nm diode laser on the functionality of platelets was evaluated to assess laser-induced changes in the mitochondria of platelets. Platelets isolated from fresh human blood was irradiated with varying fluences of low power laser (0.5, 1 and 2 J/cm²). The Mitochondrial membrane potential (MMP) of the platelets were assessed through flow cytometry using Tetramethylrhodamine, Methyl Ester (TMRM), post-irradiation.

Original language	English
Title of host publication	Frontiers in Optics, FiO 2017
Publisher	OSA - The Optical Society
Volume	Part F66-FiO 2017
ISBN (Electronic)	9781557528209
ISBN (Print)	9781943580330
DOIs	https://doi.org/10.1364/FIO.2017.JTu2A.61
Publication status	Published - 01-01-2017
Event	Frontiers in Optics, FiO 2017 - Washington, United States Duration: 18-09-2017 → 21-09-2017

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F66-FiO 2017
ISSN (Electronic)	2162-2701

Conference

Conference	Frontiers in Optics, FiO 2017
Country/Territory	United States
City	Washington
Period	18-09-17 → 21-09-17

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Mechanics of Materials

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10.1364/FIO.2017.JTu2A.61

Cite this

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title = "Photo-bio modulatory response of platelets to low power laser - A pilot study",

abstract = "Platelets initiate as well as regulate the cascade of events that take place during tissue repair through haemostatic regulation and secretion of growth factors and cytokines. In the present study the effect of 830 nm diode laser on the functionality of platelets was evaluated to assess laser-induced changes in the mitochondria of platelets. Platelets isolated from fresh human blood was irradiated with varying fluences of low power laser (0.5, 1 and 2 J/cm2). The Mitochondrial membrane potential (MMP) of the platelets were assessed through flow cytometry using Tetramethylrhodamine, Methyl Ester (TMRM), post-irradiation.",

author = "Bharath Rathnakar and Namita Bhyravbhatla and Subhash Chandra and Rao, {Bola Sadashiva Satish} and Mahato, {Krishna Kishore}",

note = "Publisher Copyright: {\textcopyright} OSA 2017.; Frontiers in Optics, FiO 2017 ; Conference date: 18-09-2017 Through 21-09-2017",

year = "2017",

month = jan,

day = "1",

doi = "10.1364/FIO.2017.JTu2A.61",

language = "English",

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Rathnakar, B, Bhyravbhatla, N, Chandra, S, Rao, BSS & Mahato, KK 2017, Photo-bio modulatory response of platelets to low power laser - A pilot study. in Frontiers in Optics, FiO 2017. vol. Part F66-FiO 2017, Optics InfoBase Conference Papers, vol. Part F66-FiO 2017, OSA - The Optical Society, Frontiers in Optics, FiO 2017, Washington, United States, 18-09-17. https://doi.org/10.1364/FIO.2017.JTu2A.61

Photo-bio modulatory response of platelets to low power laser - A pilot study. / Rathnakar, Bharath; Bhyravbhatla, Namita; Chandra, Subhash et al.
Frontiers in Optics, FiO 2017. Vol. Part F66-FiO 2017 OSA - The Optical Society, 2017. (Optics InfoBase Conference Papers; Vol. Part F66-FiO 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Photo-bio modulatory response of platelets to low power laser - A pilot study

AU - Rathnakar, Bharath

AU - Bhyravbhatla, Namita

AU - Chandra, Subhash

AU - Rao, Bola Sadashiva Satish

AU - Mahato, Krishna Kishore

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Platelets initiate as well as regulate the cascade of events that take place during tissue repair through haemostatic regulation and secretion of growth factors and cytokines. In the present study the effect of 830 nm diode laser on the functionality of platelets was evaluated to assess laser-induced changes in the mitochondria of platelets. Platelets isolated from fresh human blood was irradiated with varying fluences of low power laser (0.5, 1 and 2 J/cm2). The Mitochondrial membrane potential (MMP) of the platelets were assessed through flow cytometry using Tetramethylrhodamine, Methyl Ester (TMRM), post-irradiation.

AB - Platelets initiate as well as regulate the cascade of events that take place during tissue repair through haemostatic regulation and secretion of growth factors and cytokines. In the present study the effect of 830 nm diode laser on the functionality of platelets was evaluated to assess laser-induced changes in the mitochondria of platelets. Platelets isolated from fresh human blood was irradiated with varying fluences of low power laser (0.5, 1 and 2 J/cm2). The Mitochondrial membrane potential (MMP) of the platelets were assessed through flow cytometry using Tetramethylrhodamine, Methyl Ester (TMRM), post-irradiation.

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DO - 10.1364/FIO.2017.JTu2A.61

M3 - Conference contribution

AN - SCOPUS:85035079361

SN - 9781943580330

VL - Part F66-FiO 2017

T3 - Optics InfoBase Conference Papers

BT - Frontiers in Optics, FiO 2017

PB - OSA - The Optical Society

T2 - Frontiers in Optics, FiO 2017

Y2 - 18 September 2017 through 21 September 2017

ER -

Photo-bio modulatory response of platelets to low power laser - A pilot study

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