TY - JOUR
T1 - Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice
AU - Prabhu, Vijendra
AU - Rao, Bola Sadashiva Satish
AU - Rao, Anuradha Calicut Kini
AU - Prasad, Keerthana
AU - Mahato, Krishna Kishore
N1 - Funding Information:
Open access funding provided by Manipal Academy of Higher Education, Manipal. Life Sciences Research Board-Defence Research and Development Organization (LSRB-DRDO), G.O.I. project no. DLS/81/48222/LSRB-164/BDB/2008, entitled “Development of fiber optic probe-based low level laser therapy (LLLT) for tissue regeneration” funded this work.
Funding Information:
The authors acknowledge the help of Mr. Subhash Chandra, Junior Engineer, Department of Biophysics, Manipal School of Life Sciences (MSLS), MAHE, Manipal, during animal experiments. The authors are also thankful to Dr. K. Satyamoorthy, Professor and Director, MSLS, MAHE, Manipal, for his constant support and MAHE, Manipal, India, for providing the necessary facilities.
Publisher Copyright:
© 2020, The Author(s).
PY - 2022/2
Y1 - 2022/2
N2 - The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm2 when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm2 treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.
AB - The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm2 when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm2 treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.
UR - http://www.scopus.com/inward/record.url?scp=85096809663&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096809663&partnerID=8YFLogxK
U2 - 10.1007/s10103-020-03202-z
DO - 10.1007/s10103-020-03202-z
M3 - Article
C2 - 33247410
AN - SCOPUS:85096809663
SN - 0268-8921
VL - 37
SP - 171
EP - 180
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 1
ER -