Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter

Nirmal Mazumder; Jianjun Qiu; Matthew R. Foreman; Carlos Macías Romero; Chih Wei Hu; Han Ruei Tsai; Peter Török; Fu Jen Kao

doi:10.1364/OE.20.014090

Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter

Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Chih Wei Hu, Han Ruei Tsai, Peter Török, Fu Jen Kao

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Contribution to journal › Article › peer-review

51 Citations (SciVal)

Abstract

We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.

Original language	English
Pages (from-to)	14090-14099
Number of pages	10
Journal	Optics Express
Volume	20
Issue number	13
DOIs	https://doi.org/10.1364/OE.20.014090
Publication status	Published - 18-06-2012

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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abstract = "We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.",

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doi = "10.1364/OE.20.014090",

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AU - Foreman, Matthew R.

AU - Romero, Carlos Macías

AU - Hu, Chih Wei

AU - Tsai, Han Ruei

AU - Török, Peter

AU - Kao, Fu Jen

PY - 2012/6/18

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AB - We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.

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Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter

Abstract

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