TY - JOUR
T1 - Polyphenol stabilized copper nanoparticle formulations for rapid disinfection of bacteria and virus on diverse surfaces
AU - Sadani, Kapil
AU - Nag, Pooja
AU - Pisharody, Lakshmi
AU - Thian, Xiao Yun
AU - Bajaj, Geetika
AU - Natu, Gayatri
AU - Mukherji, Suparna
AU - Mukherji, Soumyo
N1 - Funding Information:
The authors would like to acknowledge the Sophisticated Analytical Instrument facility (SAIF), IIT Bombay for providing FEG-TEM, ICP-AES and EELS facilities and the Department of Chemical Engineering, IIT Bombay for CryoFEG TEM facility for bacteria characterization. The authors acknowledge Dr Arpan Pradhan and Prof. Dulal Panda for their help in cytotoxicity testing. The authors acknowledge Prof. Kiran Kondabagil for gifting bacteriophage phi6 and Applied Materials for funding this work.
Publisher Copyright:
© 2021 IOP Publishing Ltd Printed in the UK
PY - 2022/1/15
Y1 - 2022/1/15
N2 - Rapid and sustained disinfection of surfaces is necessary to check the spread of pathogenic microbes. The current study proposes a method of synthesis and use of copper nanoparticles (CuNPs) for contact disinfection of pathogenic microorganisms. Polyphenol stabilized CuNPs were synthesized by successive reductive disassembly and reassembly of copper phenolic complexes. Morphological and compositional characterization by transmission electron microscope (TEM), selected area diffraction and electron energy loss spectroscopy revealed monodispersed spherical (f 5-8 nm) CuNPs with coexisting Cu, Cu(I) and Cu (II) phases. Various commercial grade porous and non-porous substrates, such as, glass, stainless steel, cloth, plastic and silk were coated with the nanoparticles. Complete disinfection of 107copies of surrogate enveloped and non-enveloped viruses: bacteriophage MS2, SUSP2, phi6; and gram negative as well as gram positive bacteria: Escherichia coli and Staphylococcus aureus was achieved on most substrates within minutes. Structural cell damage was further analytically confirmed by TEM. The formulation was well retained on woven cloth surfaces even after repeated washing, thereby revealing its promising potential for use in biosafe clothing. In the face of the current pandemic, the nanomaterials developed are also of commercial utility as an eco-friendly, mass producible alternative to bleach and alcohol based public space sanitizers used today.
AB - Rapid and sustained disinfection of surfaces is necessary to check the spread of pathogenic microbes. The current study proposes a method of synthesis and use of copper nanoparticles (CuNPs) for contact disinfection of pathogenic microorganisms. Polyphenol stabilized CuNPs were synthesized by successive reductive disassembly and reassembly of copper phenolic complexes. Morphological and compositional characterization by transmission electron microscope (TEM), selected area diffraction and electron energy loss spectroscopy revealed monodispersed spherical (f 5-8 nm) CuNPs with coexisting Cu, Cu(I) and Cu (II) phases. Various commercial grade porous and non-porous substrates, such as, glass, stainless steel, cloth, plastic and silk were coated with the nanoparticles. Complete disinfection of 107copies of surrogate enveloped and non-enveloped viruses: bacteriophage MS2, SUSP2, phi6; and gram negative as well as gram positive bacteria: Escherichia coli and Staphylococcus aureus was achieved on most substrates within minutes. Structural cell damage was further analytically confirmed by TEM. The formulation was well retained on woven cloth surfaces even after repeated washing, thereby revealing its promising potential for use in biosafe clothing. In the face of the current pandemic, the nanomaterials developed are also of commercial utility as an eco-friendly, mass producible alternative to bleach and alcohol based public space sanitizers used today.
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U2 - 10.1088/1361-6528/ac2e77
DO - 10.1088/1361-6528/ac2e77
M3 - Article
AN - SCOPUS:85119213443
SN - 0957-4484
VL - 33
JO - Nanotechnology
JF - Nanotechnology
IS - 3
M1 - 035701
ER -