TY - JOUR
T1 - Polyspermic fertilisation of porcine oocytes in vitro – A current review
AU - Chundekkad, Pavitra
AU - Stankiewicz, Tomasz
AU - Blaszczyk, Barbara
AU - Udala, Jan
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Polyspermic fertilisation has been a continual problem in porcine in vitro fertilisation. Over the years, researchers have tried to resolve this persistent problem, but have not been entirely successful in their endeav-our. This can be attributed to several factors. This review discusses the role of sperm capacitation in the in vitro fertilisation and the most recent studies on the capacitation media. This includes the results obtained by testing the effect of various compounds on sperm capacitation. Additionally, the importance of the oviductal fluid on the porcine oocytes prior to fertilisation is also discussed. This is of significance, as the experiments show that oviductal fluid is crucial for the pre-fertilisation zona pellucida hardening. Also, the significance of using high quality oocytes for the in vitro fertilisation is highlighted. Lastly, the different and latest techniques to prevent or reduce polyspermic fertilisation have been reviewed.
AB - Polyspermic fertilisation has been a continual problem in porcine in vitro fertilisation. Over the years, researchers have tried to resolve this persistent problem, but have not been entirely successful in their endeav-our. This can be attributed to several factors. This review discusses the role of sperm capacitation in the in vitro fertilisation and the most recent studies on the capacitation media. This includes the results obtained by testing the effect of various compounds on sperm capacitation. Additionally, the importance of the oviductal fluid on the porcine oocytes prior to fertilisation is also discussed. This is of significance, as the experiments show that oviductal fluid is crucial for the pre-fertilisation zona pellucida hardening. Also, the significance of using high quality oocytes for the in vitro fertilisation is highlighted. Lastly, the different and latest techniques to prevent or reduce polyspermic fertilisation have been reviewed.
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U2 - 10.17221/79/2019-VETMED
DO - 10.17221/79/2019-VETMED
M3 - Review article
AN - SCOPUS:85078064686
SN - 0375-8427
VL - 64
SP - 513
EP - 520
JO - Veterinarni Medicina
JF - Veterinarni Medicina
IS - 12
ER -