Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent

K. Satyamoorthy; Manik P. Chitnis; Vathsala S. Basrur; Suresh H. Advani

doi:10.1016/0145-2126(86)90341-3

Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent

K. Satyamoorthy, Manik P. Chitnis, Vathsala S. Basrur, Suresh H. Advani

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (³H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10^-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.

Original language	English
Pages (from-to)	1327-1330
Number of pages	4
Journal	Leukemia Research
Volume	10
Issue number	11
DOIs	https://doi.org/10.1016/0145-2126(86)90341-3
Publication status	Published - 1986

All Science Journal Classification (ASJC) codes

Hematology
Oncology
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0145-2126(86)90341-3

Cite this

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title = "Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent",

abstract = "The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.",

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T1 - Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent

AU - Satyamoorthy, K.

AU - Chitnis, Manik P.

AU - Basrur, Vathsala S.

AU - Advani, Suresh H.

PY - 1986

Y1 - 1986

N2 - The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.

AB - The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.

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ER -

Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent

Abstract

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