TY - JOUR
T1 - Precocious in vitro flowering in threatened ornamental orchid, Dendrobium ovatum – Decoding the causal factors
AU - Pujari, Ipsita
AU - Sankar Babu, Vidhu
N1 - Funding Information:
The authors thank the Science and Engineering Research Board-Extra Mural Research (SERB-EMR) (presently called Core Research Grant [ CRG ]), Government of India, File No. EMR/2015/001816 for funding the research project in fellowship towards manpower, consumables, and contingency charges from 2016 to 2019. IP thanks Manipal Academy of Higher Education (MAHE), India, for providing the prestigious Dr T. M. A. Pai PhD Scholarship. IP also acknowledge SERB-EMR for granting the Junior Research Fellowship (JRF) from May 2017-December 2019.
Publisher Copyright:
© 2022
PY - 2022/9
Y1 - 2022/9
N2 - Dendrobium has been the focus of floral industries for decades. Wild Dendrobiums serve as germplasm for creating hybrids in the cut flower industry and are priced high. But, fewer reports exist on the wild tropical species of Dendrobium. The present study reports in vitro flowering in Dendrobium ovatum - an epiphytic orchid species endemic to the oriental tropics. The seedlings are raised from the protocorms and protocorm-like bodies. This research also highlights the traits displayed by the in vitro flowers of D. ovatum. An optimized modified MS medium was developed to enhance the flowering in the orchid species. The pre-treatment methods, notably dark incubation for 12 h and prechilling at 15 °C for 2 h, demonstrated the synthesis of pharmacologically relevant bioactives viz., ‘Moscatilin’ and ‘Resveratrol’. The tissue culture system devised can decode the enigmatic reproductive biology and its underlying molecular regulation of D. ovatum. This strategy could be emulated in other horticultural, medicinal and food crops for producing industrially relevant phytochemicals.
AB - Dendrobium has been the focus of floral industries for decades. Wild Dendrobiums serve as germplasm for creating hybrids in the cut flower industry and are priced high. But, fewer reports exist on the wild tropical species of Dendrobium. The present study reports in vitro flowering in Dendrobium ovatum - an epiphytic orchid species endemic to the oriental tropics. The seedlings are raised from the protocorms and protocorm-like bodies. This research also highlights the traits displayed by the in vitro flowers of D. ovatum. An optimized modified MS medium was developed to enhance the flowering in the orchid species. The pre-treatment methods, notably dark incubation for 12 h and prechilling at 15 °C for 2 h, demonstrated the synthesis of pharmacologically relevant bioactives viz., ‘Moscatilin’ and ‘Resveratrol’. The tissue culture system devised can decode the enigmatic reproductive biology and its underlying molecular regulation of D. ovatum. This strategy could be emulated in other horticultural, medicinal and food crops for producing industrially relevant phytochemicals.
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U2 - 10.1016/j.cpb.2022.100257
DO - 10.1016/j.cpb.2022.100257
M3 - Article
AN - SCOPUS:85137018912
SN - 2214-6628
VL - 31
JO - Current Plant Biology
JF - Current Plant Biology
M1 - 100257
ER -