TY - JOUR
T1 - Preparation and characterization of chitosan-gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications
AU - Peter, Mathew
AU - Ganesh, Nitya
AU - Selvamurugan, N.
AU - Nair, S. V.
AU - Furuike, T.
AU - Tamura, H.
AU - Jayakumar, R.
N1 - Funding Information:
The Department of Science and Technology, Government of India supported this work, under a centre grant of the Nanoscience and Nanotechnology Initiative program monitored by Dr. C.N.R. Rao. The authors are thankful to Prof. Greta R. Patzke, Institute of Inorganic Chemistry, University of Zurich for helping in TEM studies. The authors are also thankful to Mr. Sajin. P. Ravi for helping in SEM studies.
PY - 2010/5/5
Y1 - 2010/5/5
N2 - Chitosan is a novel biocompatible, biodegradable polymer for potential use in tissue engineering. In this work, chitosan-gelatin/nanophase hydroxyapatite composite scaffolds were prepared by blending chitosan and gelatin with nanophase hydroxyapatite (nHA). The prepared nHA was characterized using TEM, XRD and FT-IR. The prepared composite scaffolds were characterized using SEM, FT-IR and XRD studies. The composite scaffolds were highly porous with a pore size of 150-300 μm. In addition, density, swelling ratio, degradation, biomineralization, cytotoxicity and cell attachment of the composite scaffolds were studied. The scaffolds showed good swelling character, which could be modulated by varying ratio of chitosan and gelatin. Composite scaffolds in the presence of nHA showed a decreased degradation rate and increased mineralization in SBF. The biological response of MG-63 cells on nanocomposite scaffolds was superior in terms of improved cell attachment, higher proliferation, and spreading compared to chitosan-gelatin (CG) scaffold.
AB - Chitosan is a novel biocompatible, biodegradable polymer for potential use in tissue engineering. In this work, chitosan-gelatin/nanophase hydroxyapatite composite scaffolds were prepared by blending chitosan and gelatin with nanophase hydroxyapatite (nHA). The prepared nHA was characterized using TEM, XRD and FT-IR. The prepared composite scaffolds were characterized using SEM, FT-IR and XRD studies. The composite scaffolds were highly porous with a pore size of 150-300 μm. In addition, density, swelling ratio, degradation, biomineralization, cytotoxicity and cell attachment of the composite scaffolds were studied. The scaffolds showed good swelling character, which could be modulated by varying ratio of chitosan and gelatin. Composite scaffolds in the presence of nHA showed a decreased degradation rate and increased mineralization in SBF. The biological response of MG-63 cells on nanocomposite scaffolds was superior in terms of improved cell attachment, higher proliferation, and spreading compared to chitosan-gelatin (CG) scaffold.
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U2 - 10.1016/j.carbpol.2009.11.050
DO - 10.1016/j.carbpol.2009.11.050
M3 - Article
AN - SCOPUS:77949655144
SN - 0144-8617
VL - 80
SP - 687
EP - 694
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 3
ER -