TY - JOUR
T1 - Preparation and characterization of PVA/Chitosan cross-linked 3D scaffolds for liver tissue engineering
AU - Supriya Bhatt, S.
AU - Thakur, Goutam
AU - Nune, Manasa
N1 - Funding Information:
The authors would like to acknowledge the Department of Science & Technology, India for the SERB POWER grant (SPG/2021/003703) and SERB Start-up research grant (SRG/2019/002130) for the financial support and the Manipal Institute of Regenerative Medicine for the infrastructural support. We would also like to thank Dr. T.M.A. Pai Ph.D. Scholarship from Manipal Academy of Higher Education. We acknowledge Ms. Aishwarya Bose, MIT Biomedical engineering B. Tech intern for her contribution to the present work. We also would like to thank Prof. Jyothi Prasanna, MIRM for providing us with the HepG2 cells.
Publisher Copyright:
© 2023 Elsevier Ltd. All rights reserved.
PY - 2023
Y1 - 2023
N2 - In this study, chitosan, and PVA are used to fabricate scaffolds using the 3D bioprinting technique for liver tissue engineering. PVA and chitosan hydrogels are cross-linked by the glutaraldehyde, genipin, and freeze-thaw methods. Hydrogels are then characterized by different techniques such as SEM, FTIR, swelling ratio, and biodegradability tests. Further, bioink is prepared using Chitosan/PVA hydrogel and HepG2 cells. Gels cross-linked by genipin were mechanically stable and also demonstrated higher cell viability compared to gels cross-linked by other methods. Overall, the chitosan-PVA cross-linked 3D hydrogels indicated substantial promise for liver tissue engineering.
AB - In this study, chitosan, and PVA are used to fabricate scaffolds using the 3D bioprinting technique for liver tissue engineering. PVA and chitosan hydrogels are cross-linked by the glutaraldehyde, genipin, and freeze-thaw methods. Hydrogels are then characterized by different techniques such as SEM, FTIR, swelling ratio, and biodegradability tests. Further, bioink is prepared using Chitosan/PVA hydrogel and HepG2 cells. Gels cross-linked by genipin were mechanically stable and also demonstrated higher cell viability compared to gels cross-linked by other methods. Overall, the chitosan-PVA cross-linked 3D hydrogels indicated substantial promise for liver tissue engineering.
UR - https://www.scopus.com/pages/publications/85149955779
UR - https://www.scopus.com/inward/citedby.url?scp=85149955779&partnerID=8YFLogxK
U2 - 10.1016/j.matpr.2023.02.251
DO - 10.1016/j.matpr.2023.02.251
M3 - Article
AN - SCOPUS:85149955779
SN - 2214-7853
JO - Materials Today: Proceedings
JF - Materials Today: Proceedings
ER -