Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy

Surekha Barkur, Aseefhali Bankapur, Madhura Pradhan, Santhosh Chidangil, Deepak Mathur, Uma Ladiwala

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

Original languageEnglish
Article number085001
JournalJournal of Biomedical Optics
Issue number8
Publication statusPublished - 01-08-2015

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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