Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Sujatha; Lavanya Rai; Pratap Kumar; B. R. Krishnanand; K. K. Mahato; Sajan D. George; V. B. Kartha; C. Santhosh

doi:10.1117/12.699711

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Sujatha, Lavanya Rai, Pratap Kumar, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, C. Santhosh

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Volume	6441
DOIs	https://doi.org/10.1117/12.699711
Publication status	Published - 2007
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: 22-01-2007 → 24-01-2007

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country/Territory	United States
City	San Jose, CA
Period	22-01-07 → 24-01-07

All Science Journal Classification (ASJC) codes

Engineering(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.699711

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Sujatha, Rai, L., Kumar, P., Krishnanand, B. R., Mahato, K. K., George, S. D., Kartha, V. B., & Santhosh, C. (2007). Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V (Vol. 6441). [64410B] https://doi.org/10.1117/12.699711

@inproceedings{748af8e9e63041a8aa4a034bf582e8d1,

title = "Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence",

abstract = "HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.",

author = "Sujatha and Lavanya Rai and Pratap Kumar and Krishnanand, {B. R.} and Mahato, {K. K.} and George, {Sajan D.} and Kartha, {V. B.} and C. Santhosh",

year = "2007",

doi = "10.1117/12.699711",

language = "English",

isbn = "0819465542",

volume = "6441",

booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V",

note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V ; Conference date: 22-01-2007 Through 24-01-2007",

}

Sujatha, Rai, L, Kumar, P, Krishnanand, BR, Mahato, KK , George, SD, Kartha, VB & Santhosh, C 2007, Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V. vol. 6441, 64410B, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, San Jose, CA, United States, 22-01-07. https://doi.org/10.1117/12.699711

TY - GEN

T1 - Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

AU - Sujatha,

AU - Rai, Lavanya

AU - Kumar, Pratap

AU - Krishnanand, B. R.

AU - Mahato, K. K.

AU - George, Sajan D.

AU - Kartha, V. B.

AU - Santhosh, C.

PY - 2007

Y1 - 2007

N2 - HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

AB - HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

UR - http://www.scopus.com/inward/record.url?scp=34247500445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247500445&partnerID=8YFLogxK

U2 - 10.1117/12.699711

DO - 10.1117/12.699711

M3 - Conference contribution

AN - SCOPUS:34247500445

SN - 0819465542

SN - 9780819465542

VL - 6441

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

Y2 - 22 January 2007 through 24 January 2007

ER -

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

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UN SDGs

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