Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Sujatha Sujatha; Lavanya Rai; Pratap Kumar; B. R. Krishnanand; K. K. Mahato; Sajan D. George; V. B. Kartha; C. Santhosh

doi:10.1117/12.699711

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Sujatha
, Lavanya Rai
, Pratap Kumar
, B. R. Krishnanand
, K. K. Mahato
, Sajan D. George
, V. B. Kartha
, C. Santhosh

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Volume	6441
DOIs	https://doi.org/10.1117/12.699711
Publication status	Published - 2007
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: 22-01-2007 → 24-01-2007

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6441
ISSN (Print)	1605-7422

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country/Territory	United States
City	San Jose, CA
Period	22-01-07 → 24-01-07

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

General Engineering

Access to Document

10.1117/12.699711

Cite this

Sujatha, Rai, L., Kumar, P., Krishnanand, B. R., Mahato, K. K., George, S. D., Kartha, V. B., & Santhosh, C. (2007). Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V (Vol. 6441). Article 64410B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6441). https://doi.org/10.1117/12.699711

@inproceedings{748af8e9e63041a8aa4a034bf582e8d1,

title = "Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence",

abstract = "HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.",

author = "Sujatha and Lavanya Rai and Pratap Kumar and Krishnanand, \{B. R.\} and Mahato, \{K. K.\} and George, \{Sajan D.\} and Kartha, \{V. B.\} and C. Santhosh",

year = "2007",

doi = "10.1117/12.699711",

language = "English",

isbn = "0819465542",

volume = "6441",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V",

note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V ; Conference date: 22-01-2007 Through 24-01-2007",

}

Sujatha, Rai, L, Kumar, P, Krishnanand, BR, Mahato, KK , George, SD, Kartha, VB & Santhosh, C 2007, Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V. vol. 6441, 64410B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, San Jose, CA, United States, 22-01-07. https://doi.org/10.1117/12.699711

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. / Sujatha; Rai, Lavanya; Kumar, Pratap et al.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V. Vol. 6441 2007. 64410B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6441).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

AU - Sujatha, null

AU - Rai, Lavanya

AU - Kumar, Pratap

AU - Krishnanand, B. R.

AU - Mahato, K. K.

AU - George, Sajan D.

AU - Kartha, V. B.

AU - Santhosh, C.

PY - 2007

Y1 - 2007

N2 - HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

AB - HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

UR - https://www.scopus.com/pages/publications/34247500445

UR - https://www.scopus.com/pages/publications/34247500445#tab=citedBy

U2 - 10.1117/12.699711

DO - 10.1117/12.699711

M3 - Conference contribution

AN - SCOPUS:34247500445

SN - 0819465542

SN - 9780819465542

VL - 6441

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

Y2 - 22 January 2007 through 24 January 2007

ER -

Sujatha, Rai L, Kumar P, Krishnanand BR, Mahato KK , George SD et al. Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V. Vol. 6441. 2007. 64410B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.699711

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography- laser induced fluorescence

Abstract

Publication series

Conference

UN SDGs

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this