Proteinaceous α-amylase inhibitors of Setaria italica Linn (Co-6) and its effect on α-amylase from human saliva and Bacillus sp

Vinoth Kumar Thirumalairaj; Pamala Auxlin Anitha; Geetharamani Durairaj; Sudha G. Menon; Lakshmanasenthil Shanmugaasokan

doi:10.7324/JAPS.2014.41205

Proteinaceous α-amylase inhibitors of Setaria italica Linn (Co-6) and its effect on α-amylase from human saliva and Bacillus sp

Vinoth Kumar Thirumalairaj^*, Pamala Auxlin Anitha, Geetharamani Durairaj, Sudha G. Menon, Lakshmanasenthil Shanmugaasokan

^*Corresponding author for this work

Department of Ophthalmology, Kasturba Medical College, Manipal

Research output: Contribution to journal › Article › peer-review

Abstract

The specific a-amylase inhibitory activity of Setaria italica Linn (Co-6) was determined by using heat-treated and ammonium sulphate precipitated fractions of α-amylase isolated from Bacillus sp. The a-amylase inhibitor from S. italica Linn (Co-6) was precipitated with 1.0 M fraction of ammonium sulphate and used for further purification and characterization. To overcome the endogenous a-amylase activity, the extract was subjected to the heat treatment at 60 °C. Complete loss of a-amylase inhibitory activity of the millet extract was evidenced above 85 °C. The results of this study suggest that, the use of S. italica Linn (Co-6) flour exhibits significant α- amylase inhibitory activity, in a dose dependent manner for Non- Insulin Dependent Diabetes Mellitus (NIDDM).

Original language	English
Pages (from-to)	26-29
Number of pages	4
Journal	Journal of Applied Pharmaceutical Science
Volume	4
Issue number	12
DOIs	https://doi.org/10.7324/JAPS.2014.41205
Publication status	Published - 2014

All Science Journal Classification (ASJC) codes

Medicine (miscellaneous)
General Pharmacology, Toxicology and Pharmaceutics
Pharmacology (medical)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.7324/JAPS.2014.41205

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title = "Proteinaceous α-amylase inhibitors of Setaria italica Linn (Co-6) and its effect on α-amylase from human saliva and Bacillus sp",

abstract = "The specific a-amylase inhibitory activity of Setaria italica Linn (Co-6) was determined by using heat-treated and ammonium sulphate precipitated fractions of α-amylase isolated from Bacillus sp. The a-amylase inhibitor from S. italica Linn (Co-6) was precipitated with 1.0 M fraction of ammonium sulphate and used for further purification and characterization. To overcome the endogenous a-amylase activity, the extract was subjected to the heat treatment at 60 °C. Complete loss of a-amylase inhibitory activity of the millet extract was evidenced above 85 °C. The results of this study suggest that, the use of S. italica Linn (Co-6) flour exhibits significant α- amylase inhibitory activity, in a dose dependent manner for Non- Insulin Dependent Diabetes Mellitus (NIDDM).",

author = "Thirumalairaj, \{Vinoth Kumar\} and Anitha, \{Pamala Auxlin\} and Geetharamani Durairaj and Menon, \{Sudha G.\} and Lakshmanasenthil Shanmugaasokan",

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doi = "10.7324/JAPS.2014.41205",

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AU - Thirumalairaj, Vinoth Kumar

AU - Anitha, Pamala Auxlin

AU - Durairaj, Geetharamani

AU - Menon, Sudha G.

AU - Shanmugaasokan, Lakshmanasenthil

PY - 2014

Y1 - 2014

N2 - The specific a-amylase inhibitory activity of Setaria italica Linn (Co-6) was determined by using heat-treated and ammonium sulphate precipitated fractions of α-amylase isolated from Bacillus sp. The a-amylase inhibitor from S. italica Linn (Co-6) was precipitated with 1.0 M fraction of ammonium sulphate and used for further purification and characterization. To overcome the endogenous a-amylase activity, the extract was subjected to the heat treatment at 60 °C. Complete loss of a-amylase inhibitory activity of the millet extract was evidenced above 85 °C. The results of this study suggest that, the use of S. italica Linn (Co-6) flour exhibits significant α- amylase inhibitory activity, in a dose dependent manner for Non- Insulin Dependent Diabetes Mellitus (NIDDM).

AB - The specific a-amylase inhibitory activity of Setaria italica Linn (Co-6) was determined by using heat-treated and ammonium sulphate precipitated fractions of α-amylase isolated from Bacillus sp. The a-amylase inhibitor from S. italica Linn (Co-6) was precipitated with 1.0 M fraction of ammonium sulphate and used for further purification and characterization. To overcome the endogenous a-amylase activity, the extract was subjected to the heat treatment at 60 °C. Complete loss of a-amylase inhibitory activity of the millet extract was evidenced above 85 °C. The results of this study suggest that, the use of S. italica Linn (Co-6) flour exhibits significant α- amylase inhibitory activity, in a dose dependent manner for Non- Insulin Dependent Diabetes Mellitus (NIDDM).

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Proteinaceous α-amylase inhibitors of Setaria italica Linn (Co-6) and its effect on α-amylase from human saliva and Bacillus sp

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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