Quantification of domperidone, paracetamol, esomeprazole and lansoprazole in pharmaceutical dosage forms by reversed phase high performance liquid chromatography

A simple, sensitive, and accurate high performance liquid chromatographic method developed and validated for the simultaneous estimation of domperidone, paracetamol, esomeprazole, and lansoprazole in pharmaceutical dosage forms.The assay involved the use of photo diode array detection at 285 nm for domperidone, 246 nm for paracetamol, 300 nm for esomeprazole, and 283 nm for lansoprazole. The compounds were well separated on a Supelco C18 (250×4.6mm,5μ) reversed-phase column and mobile phase consisting of 0.01 M, pH 7.00, dipotassium hydrogen phosphate buffer - acetonitrile (65:35 v/v) at a flow rate of 1.0 mL min^-1. The linearity range was 0.5 - 50 μg mL^-1 for all the four analytes domperidone, paracetamol, esomeprazole and lansoprazole. Limits of detection (LOD) obtained were 0.1 μg mL^-1 for each of the four drugs. The developed analytical method was validated as per ICHQ2 (R1) guidance. Study showed that reversed-phase liquid chromatography is sensitive and selective for the determination of domperidone, paracetamol, esomeprazole, and lansoprazole in pharmaceutical dosage forms.