TY - JOUR
T1 - Quantitative Analysis of Phyllanthus amarus and Phyllanthus urinaria for Bioactive Molecules using High-Pressure Liquid Chromatography and Liquid Chromatography–Mass Spectrometry
AU - Annamalai, Muthusamy
AU - Sanjay, ER
AU - Nagendra Prasad, HN
AU - Radhakrishna Rao, M.
AU - Joshi, Manjunath B
AU - Rai, Dr. Padmalatha S
AU - Kapaettu, Satyamoorthy
PY - 2017
Y1 - 2017
N2 - Phyllanthus amarus Schum. & Thonn. and P.
urinaria Linn. are valuable medicinal plants which are
widely used in several countries for the treatment of
jaundice, diabetes, cancer and other important diseases.
The Phyllanthus species are well-known for their important
bioactive lignans (phyllanthin, hypophyllanthin and niranthin)
and an antioxidant (ellagic acid). Qualitative and
quantitative analyses by high performance liquid chromatography
(HPLC) and liquid chromatography coupled to
mass spectrometry (LC–MS) method revealed the contents
of four bioactive molecules in Phyllanthus amarus and
Phyllanthus urinaria from district Udupi, Karnataka, India.
The phyllanthin, hypophyllanthin and niranthin were eluted
out at specific retention time of 16.008, 14.838 and
21.106 min respectively by isocratic method and ellagic
acid was eluted out at 28.656 min using gradient method in
HPLC. The extracts of P. amarus from three taluks in
district Udupi, Karnataka, India showed higher amounts of
phyllanthin, hypophyllanthin and niranthin as compared to
that of P. urinaria. The ellagic acid was found to be
quantitatively higher than the three lignans of P. amarus
and P. urinaria. The phytochemical profiling of bioactive
compounds in these two species collected from district
Udupi showed significant variations in their contents. The
quantification of bioactive molecules using HPLC and LC–
MS is consistent and reproducible for the future studies on
somaclonal variant plants with varied amount of bioactive
molecules, and transgenic plants with over-expression of
lignans and phenolic compounds which has higher market
value in the pharmaceutical industry.
AB - Phyllanthus amarus Schum. & Thonn. and P.
urinaria Linn. are valuable medicinal plants which are
widely used in several countries for the treatment of
jaundice, diabetes, cancer and other important diseases.
The Phyllanthus species are well-known for their important
bioactive lignans (phyllanthin, hypophyllanthin and niranthin)
and an antioxidant (ellagic acid). Qualitative and
quantitative analyses by high performance liquid chromatography
(HPLC) and liquid chromatography coupled to
mass spectrometry (LC–MS) method revealed the contents
of four bioactive molecules in Phyllanthus amarus and
Phyllanthus urinaria from district Udupi, Karnataka, India.
The phyllanthin, hypophyllanthin and niranthin were eluted
out at specific retention time of 16.008, 14.838 and
21.106 min respectively by isocratic method and ellagic
acid was eluted out at 28.656 min using gradient method in
HPLC. The extracts of P. amarus from three taluks in
district Udupi, Karnataka, India showed higher amounts of
phyllanthin, hypophyllanthin and niranthin as compared to
that of P. urinaria. The ellagic acid was found to be
quantitatively higher than the three lignans of P. amarus
and P. urinaria. The phytochemical profiling of bioactive
compounds in these two species collected from district
Udupi showed significant variations in their contents. The
quantification of bioactive molecules using HPLC and LC–
MS is consistent and reproducible for the future studies on
somaclonal variant plants with varied amount of bioactive
molecules, and transgenic plants with over-expression of
lignans and phenolic compounds which has higher market
value in the pharmaceutical industry.
U2 - DOI 10.1007/s40011-017-0839-y
DO - DOI 10.1007/s40011-017-0839-y
M3 - Article
SN - 0369-8211
JO - Proceedings of the National Academy of Sciences India Section B - Biological Sciences
JF - Proceedings of the National Academy of Sciences India Section B - Biological Sciences
ER -
