TY - JOUR
T1 - Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence
AU - Kumari, Sandhya
AU - Salian, Sujith Raj
AU - Rao, Arpitha
AU - Somagond, Shilpa M.
AU - Kamble, Ravindra R.
AU - Nesaragi, Aravind
AU - Das, Jyotirekha
AU - Rajanikant, G. K.
AU - Mutalik, Srinivas
AU - Raghu, Shamprasad Varija
AU - Adiga, Satish Kumar
AU - Kalthur, Guruprasad
N1 - Funding Information:
This study received partial financial support from Kasturba Medical College, Manipal, Seed Money Grant (ID: 00000205). Authors SMS and RRK acknowledge the UGC, New Delhi for providing financial support under UGC-UPE program on “Antitumor activity and integrated approach” vide No. 14-3/2012 (NS/PE), dated: 14.03.2012.
Publisher Copyright:
© 2020, The Author(s).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressive sperm motility enhancement properties were further assessed for their effect on functional competence of human spermatozoa. To determine the effect on the fertilizing ability of spermatozoa processed with quinoline derivatives and to assess developmental competence of embryos derived, in vitro fertilization (IVF) was performed using mouse model. Among the nine quinoline derivatives, 2 compounds (6MQT and 2,6DQT) exhibited significant enhancement in sperm progressive motility and survival at 24 h. Further, non-significant increase in curvilinear velocity (VCL), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) was observed. Capacitation, intracellular cAMP level and tyrosine phosphorylated sperm proteins were significantly higher in 6MQT (P < 0.05) and 2,6DQT (P < 0.001) compared to control. In vitro fertilization (IVF) experiments using Swiss albino mice revealed that spermatozoa processed with 6MQT had non-significantly higher blastocyst rate and a superior blastocyst quality, while, 2,6DQT resulted in significantly lower blastocyst rate (P < 0.05) compared to control. Quinoline derivative 6MQT has significant motility enhancement property under in vitro conditions. [Figure not available: see fulltext.].
AB - In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressive sperm motility enhancement properties were further assessed for their effect on functional competence of human spermatozoa. To determine the effect on the fertilizing ability of spermatozoa processed with quinoline derivatives and to assess developmental competence of embryos derived, in vitro fertilization (IVF) was performed using mouse model. Among the nine quinoline derivatives, 2 compounds (6MQT and 2,6DQT) exhibited significant enhancement in sperm progressive motility and survival at 24 h. Further, non-significant increase in curvilinear velocity (VCL), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) was observed. Capacitation, intracellular cAMP level and tyrosine phosphorylated sperm proteins were significantly higher in 6MQT (P < 0.05) and 2,6DQT (P < 0.001) compared to control. In vitro fertilization (IVF) experiments using Swiss albino mice revealed that spermatozoa processed with 6MQT had non-significantly higher blastocyst rate and a superior blastocyst quality, while, 2,6DQT resulted in significantly lower blastocyst rate (P < 0.05) compared to control. Quinoline derivative 6MQT has significant motility enhancement property under in vitro conditions. [Figure not available: see fulltext.].
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U2 - 10.1007/s43032-020-00382-5
DO - 10.1007/s43032-020-00382-5
M3 - Article
AN - SCOPUS:85096625649
SN - 1933-7191
VL - 28
SP - 1316
EP - 1332
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 5
ER -