Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

Surekha Barkur; Aseefhali Bankapur; Santhosh Chidangil

doi:10.1117/12.2248461

Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

Surekha Barkur, Aseefhali Bankapur, Santhosh Chidangil

Department of Atomic and Molecular Physics, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm^-1, 1224 cm^-1, 1375 cm^-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

Original language	English
Title of host publication	Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
Publisher	SPIE
Volume	10150
ISBN (Electronic)	9781510607514
DOIs	https://doi.org/10.1117/12.2248461
Publication status	Published - 01-01-2016
Event	2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 - Legian - Kuta, Bali, Indonesia Duration: 24-08-2016 → 25-08-2016

Conference

Conference	2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016
Country/Territory	Indonesia
City	Legian - Kuta, Bali
Period	24-08-16 → 25-08-16

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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Cite this

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title = "Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis",

abstract = "Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.",

author = "Surekha Barkur and Aseefhali Bankapur and Santhosh Chidangil",

year = "2016",

month = jan,

day = "1",

doi = "10.1117/12.2248461",

language = "English",

volume = "10150",

booktitle = "Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016",

publisher = "SPIE",

address = "United States",

note = "2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016 ; Conference date: 24-08-2016 Through 25-08-2016",

}

Barkur, S, Bankapur, A & Chidangil, S 2016, Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis. in Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016. vol. 10150, 1015016, SPIE, 2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016, Legian - Kuta, Bali, Indonesia, 24-08-16. https://doi.org/10.1117/12.2248461

TY - GEN

T1 - Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

AU - Barkur, Surekha

AU - Bankapur, Aseefhali

AU - Chidangil, Santhosh

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

AB - Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

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DO - 10.1117/12.2248461

M3 - Conference contribution

AN - SCOPUS:85007207149

VL - 10150

BT - Second International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016

PB - SPIE

T2 - 2nd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2016

Y2 - 24 August 2016 through 25 August 2016

ER -

Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

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