TY - JOUR
T1 - Rapid high-performance thin layer chromatographic quantitative estimation of caffeine in various foods and beverages
AU - Raj, Rohit
AU - Chandrashekar, K. S.
AU - Biswas, Rounak
AU - Pai, Aravind
AU - Pai, Vasudev
N1 - Funding Information:
The authors are thankful to the Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences and Manipal Academy of Higher Education for providing laboratory facilities and other infrastructure to carry out the work.
Publisher Copyright:
© 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Caffeine is an alkaloid and well-known CNS stimulant usually added in most foods and beverages. Caffeine had drawn more attention in many nutritional products due to its stimulant effect on CNS, so that the product to which caffeine is added becomes more popular in the market. The present work was carried out to extract and estimate the amount of caffeine in the various food and beverages by chromatography technique using High-Performance Thin Layer Chromatography. Procured marketed products like confectioneries, foods and beverages are subjected for extraction and estimated the caffeine content by HPTLC method by using mobile phase Ethyl acetate: Methanol (9: 1) and quantified. Monster and Tzinga both energy drinks showed the content within the label claim (23.96 and 25.43% respectively). However other products though they did not have a label claim, showed significant caffeine content, in that dark chocolate showed the highest 11.40%, whereas diet coke did not show any caffeine content. The consumable food products like biscuits, energy drinks, and other beverages with added caffeine should have quantitative labeling on the wrapper to help the consumer to know about the caffeine. But label claim of caffeine is missing on some products, HPTLC method helps to estimate the caffeine content from the marketed products.
AB - Caffeine is an alkaloid and well-known CNS stimulant usually added in most foods and beverages. Caffeine had drawn more attention in many nutritional products due to its stimulant effect on CNS, so that the product to which caffeine is added becomes more popular in the market. The present work was carried out to extract and estimate the amount of caffeine in the various food and beverages by chromatography technique using High-Performance Thin Layer Chromatography. Procured marketed products like confectioneries, foods and beverages are subjected for extraction and estimated the caffeine content by HPTLC method by using mobile phase Ethyl acetate: Methanol (9: 1) and quantified. Monster and Tzinga both energy drinks showed the content within the label claim (23.96 and 25.43% respectively). However other products though they did not have a label claim, showed significant caffeine content, in that dark chocolate showed the highest 11.40%, whereas diet coke did not show any caffeine content. The consumable food products like biscuits, energy drinks, and other beverages with added caffeine should have quantitative labeling on the wrapper to help the consumer to know about the caffeine. But label claim of caffeine is missing on some products, HPTLC method helps to estimate the caffeine content from the marketed products.
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U2 - 10.31788/RJC.2021.1415991
DO - 10.31788/RJC.2021.1415991
M3 - Article
AN - SCOPUS:85100768932
SN - 0974-1496
VL - 14
SP - 221
EP - 226
JO - Rasayan Journal of Chemistry
JF - Rasayan Journal of Chemistry
IS - 1
ER -