TY - JOUR
T1 - Real-time and rapid detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance setup
AU - Lukose, Jijo
AU - Shetty, Vignesh
AU - Ballal, Mamatha
AU - Chidangil, Santhosh
AU - Sinha, Rajeev K.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and real-time detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ∼1.5 × 108 and ∼1 × 106 cfu ml-1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6-7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.
AB - Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and real-time detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ∼1.5 × 108 and ∼1 × 106 cfu ml-1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6-7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.
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U2 - 10.1088/1612-202X/aabed8
DO - 10.1088/1612-202X/aabed8
M3 - Article
AN - SCOPUS:85048361096
SN - 1612-2011
VL - 15
JO - Laser Physics Letters
JF - Laser Physics Letters
IS - 7
M1 - 075701
ER -