Red blood cells under externally induced stressors probed by micro-Raman spectroscopy

Jijo Lukose; Shamee Shastry; Ganesh Mohan; Santhosh Chidangil

doi:10.1016/B978-0-12-823971-1.00011-8

Red blood cells under externally induced stressors probed by micro-Raman spectroscopy

Jijo Lukose, Shamee Shastry, Ganesh Mohan, Santhosh Chidangil

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Optical techniques are becoming increasingly popular for the analysis of body fluids, particularly so after the onset of the COVID-19 pandemic. Raman spectroscopy has found special significance among them due to its ability to perform label-free investigations of biological solutions with high sensitivity and specificity. The integration of Raman spectroscopy with optical tweezers-Raman Tweezers-has been explored in conducting biochemical investigations on individual red blood cells. We present in this chapter an evaluation of various stress agents, such as intravenous fluids, certain chemicals, and metal nanoparticles, on live, human red blood cells using the Raman Tweezers technique. The technique found efficacy in monitoring hemoglobin deoxygenation, heme aggregation, heme degradation, and membrane damage in red blood cells under the influence of exogenous agents.

Original language	English
Title of host publication	Nanotechnology for Hematology, Blood Transfusion, and Artificial Blood
Publisher	Elsevier
Pages	141-165
Number of pages	25
ISBN (Electronic)	9780128239711
DOIs	https://doi.org/10.1016/B978-0-12-823971-1.00011-8
Publication status	Published - 01-01-2021

All Science Journal Classification (ASJC) codes

Engineering(all)
Materials Science(all)

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10.1016/B978-0-12-823971-1.00011-8

Cite this

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Red blood cells under externally induced stressors probed by micro-Raman spectroscopy

Abstract

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