Regioselective reaction: synthesis and biological properties of some novel mannich bases incorporating arylnitro thiophene moiety

The Mannich reaction of 3-(substituted)-4-[5-(p-nitrophenyl)-2- thienylidene] amino- 5-mercapto-1,2,4-triazoles (3) with formaldehyde and appropriate amine resulted in the regioselective formation of 1-aminomethyl-3-(substituted)-4-[5-(p-nitrophenyl)-2- thienylidene] amino-1,2,4-triazole-5-thiones (4). The structures of the new compounds were established on the basis of analytical and spectral data. The new compounds were tested for their antibacterial and antifungal activities. Most of the tested compounds showed significant antifungal activity, much higher than the standard.