TY - GEN
T1 - Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice
AU - Rathnakar, Bharath
AU - Prabhu, Vijendra
AU - Rao, Bola Sadashiva Satish
AU - Chandra, Subhash
AU - Rai, Sharada
AU - Mahato, Krishna Kishore
N1 - Publisher Copyright:
© 2016 SPIE.
PY - 2016
Y1 - 2016
N2 - The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.
AB - The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm2 (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.
UR - https://www.scopus.com/pages/publications/85011396266
UR - https://www.scopus.com/pages/publications/85011396266#tab=citedBy
U2 - 10.1117/12.2242919
DO - 10.1117/12.2242919
M3 - Conference contribution
AN - SCOPUS:85011396266
VL - 10013
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - SPIE BioPhotonics Australasia
A2 - Goldys, Ewa M.
A2 - Hutchinson, Mark R.
PB - SPIE
T2 - SPIE BioPhotonics Australasia
Y2 - 17 October 2016 through 19 October 2016
ER -