TY - JOUR
T1 - Residual gas analyzer mass spectrometry for human breath analysis
T2 - A new tool for the non-invasive diagnosis of Helicobacter pylori infection
AU - Maity, Abhijit
AU - Banik, Gourab D.
AU - Ghosh, Chiranjit
AU - Som, Suman
AU - Chaudhuri, Sujit
AU - Daschakraborty, Sunil B.
AU - Ghosh, Shibendu
AU - Ghosh, Barnali
AU - Raychaudhuri, Arup K.
AU - Pradhan, Manik
PY - 2014/3
Y1 - 2014/3
N2 - A residual gas analyzer (RGA) coupled with a high vacuum chamber is described for the non-invasive diagnosis of the Helicobacter pylori (H. pylori) infection through 13C-urea breath analysis. The present RGA-based mass spectrometry (MS) method is capable of measuring high-precision 13CO2 isotope enrichments in exhaled breath samples from individuals harboring the H. pylori infection. The system exhibited 100% diagnostic sensitivity, and 93% specificity alongside positive and negative predictive values of 95% and 100%, respectively, compared with invasive endoscopy-based biopsy tests. A statistically sound diagnostic cut-off value for the presence of H. pylori was determined to be 3.0‰ using a receiver operating characteristic curve analysis. The diagnostic accuracy and validity of the results are also supported by optical off-axis integrated cavity output spectroscopy measurements. The δ13DOBC‰ values of both methods correlated well (R2 = 0.9973 at 30 min). The RGA-based instrumental setup described here is simple, robust, easy-to-use and more portable and cost-effective compared to all other currently available detection methods, thus making it a new point-of-care medical diagnostic tool for the purpose of large-scale screening of the H. pylori infection in real time. The RGA-MS technique should have broad applicability for 13C-breath tests in a wide range of biomedical research and clinical diagnostics for many other diseases and metabolic disorders.
AB - A residual gas analyzer (RGA) coupled with a high vacuum chamber is described for the non-invasive diagnosis of the Helicobacter pylori (H. pylori) infection through 13C-urea breath analysis. The present RGA-based mass spectrometry (MS) method is capable of measuring high-precision 13CO2 isotope enrichments in exhaled breath samples from individuals harboring the H. pylori infection. The system exhibited 100% diagnostic sensitivity, and 93% specificity alongside positive and negative predictive values of 95% and 100%, respectively, compared with invasive endoscopy-based biopsy tests. A statistically sound diagnostic cut-off value for the presence of H. pylori was determined to be 3.0‰ using a receiver operating characteristic curve analysis. The diagnostic accuracy and validity of the results are also supported by optical off-axis integrated cavity output spectroscopy measurements. The δ13DOBC‰ values of both methods correlated well (R2 = 0.9973 at 30 min). The RGA-based instrumental setup described here is simple, robust, easy-to-use and more portable and cost-effective compared to all other currently available detection methods, thus making it a new point-of-care medical diagnostic tool for the purpose of large-scale screening of the H. pylori infection in real time. The RGA-MS technique should have broad applicability for 13C-breath tests in a wide range of biomedical research and clinical diagnostics for many other diseases and metabolic disorders.
UR - https://www.scopus.com/pages/publications/84899566221
UR - https://www.scopus.com/inward/citedby.url?scp=84899566221&partnerID=8YFLogxK
U2 - 10.1088/1752-7155/8/1/016005
DO - 10.1088/1752-7155/8/1/016005
M3 - Article
C2 - 24566134
AN - SCOPUS:84899566221
SN - 1752-7155
VL - 8
JO - Journal of Breath Research
JF - Journal of Breath Research
IS - 1
M1 - 016005
ER -