TY - JOUR
T1 - Reticulum vs inclusions
T2 - A learning experience in haemoglobin H disease
AU - Sridevi, H. B.
AU - Hegde, Anupapama
AU - Prashantha, B.
AU - Khadilkar, Urmila N.
AU - Chakraborti, Shrijeet
N1 - Publisher Copyright:
© 2015, Journal of Clinical and Diagnostic Research. All Rights Reserved.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Haemoglobin H disease, also known as the alpha-thalassaemia is characterized by the presence of HbH inclusions in red blood cells, detectable on supra-vital stain. We present a case of a previously asymptomatic 31-year-old male, who insidiously developed anaemia and had prominent splenomegaly. Peripheral smear examination revealed microcytic hypochromic anaemia with numerous spherocytes and moderate polychromasia. In reticulocyte preparation with Brilliant cresyl blue, HbH inclusions were mistakenly identified as granulofilamentous reticulum of reticulocytes, giving a spuriously high reticulocyte percentage. After the literature review, repeat assessment was performed and with the aid of high performance liquid chromatography result, it was possible to delineate the HbH inclusions.
AB - Haemoglobin H disease, also known as the alpha-thalassaemia is characterized by the presence of HbH inclusions in red blood cells, detectable on supra-vital stain. We present a case of a previously asymptomatic 31-year-old male, who insidiously developed anaemia and had prominent splenomegaly. Peripheral smear examination revealed microcytic hypochromic anaemia with numerous spherocytes and moderate polychromasia. In reticulocyte preparation with Brilliant cresyl blue, HbH inclusions were mistakenly identified as granulofilamentous reticulum of reticulocytes, giving a spuriously high reticulocyte percentage. After the literature review, repeat assessment was performed and with the aid of high performance liquid chromatography result, it was possible to delineate the HbH inclusions.
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U2 - 10.7860/JCDR/2015/13649.6657
DO - 10.7860/JCDR/2015/13649.6657
M3 - Article
AN - SCOPUS:84943274160
SN - 2249-782X
VL - 9
SP - ED17-ED19
JO - Journal of Clinical and Diagnostic Research
JF - Journal of Clinical and Diagnostic Research
IS - 10
ER -