TY - JOUR
T1 - Role of ERK1/2 signaling in dengue virus-induced liver injury
AU - Sreekanth, Gopinathan Pillai
AU - Chuncharunee, Aporn
AU - Sirimontaporn, Aunchalee
AU - Panaampon, Jutatip
AU - Srisawat, Chatchawan
AU - Morchang, Atthapan
AU - Malakar, Shilu
AU - Thuwajit, Peti
AU - Kooptiwut, Suwattanee
AU - Suttitheptumrong, Aroonroong
AU - Songprakhon, Pucharee
AU - Noisakran, Sansanee
AU - Yenchitsomanus, Pa thai
AU - Limjindaporn, Thawornchai
PY - 2014/8/8
Y1 - 2014/8/8
N2 - The liver is considered to be an important organ of dengue virus (DENV) replication and pathogenesis. However, molecular mechanisms of hepatic injury are still poorly understood. Modulation of Mitogen Activated Protein Kinases (MAPKs) was previously shown to affect DENV-induced apoptosis of hepatocytes in vitro. However, the in vivo role of ERK1/2, a member of the MAPK family, and the question whether its activation can facilitate cell survival or cell death, has not been thoroughly investigated. Therefore, the role of ERK1/2 in a mouse model of DENV infection was examined. Our results show that DENV induces phosphorylation of ERK1/2 and increases apoptosis. Inhibition of phosphorylated ERK1/2 by the selective ERK1/2 inhibitor, FR180204, limits hepatocyte apoptosis and reduces DENV-induced liver injury. Clinical parameters, including leucopenia, thrombocytopenia, transaminases and histology, show improvements after FR180204 treatment. The expression of cell death genes was further identified using real-time PCR array and Western blot analysis. Caspase-3 was significantly decreased in FR180204 treated DENV-infected mice compared to the levels of untreated DENV-infected mice suggesting the role of ERK1/2 signaling in immune-mediated liver injury during DENV infection.
AB - The liver is considered to be an important organ of dengue virus (DENV) replication and pathogenesis. However, molecular mechanisms of hepatic injury are still poorly understood. Modulation of Mitogen Activated Protein Kinases (MAPKs) was previously shown to affect DENV-induced apoptosis of hepatocytes in vitro. However, the in vivo role of ERK1/2, a member of the MAPK family, and the question whether its activation can facilitate cell survival or cell death, has not been thoroughly investigated. Therefore, the role of ERK1/2 in a mouse model of DENV infection was examined. Our results show that DENV induces phosphorylation of ERK1/2 and increases apoptosis. Inhibition of phosphorylated ERK1/2 by the selective ERK1/2 inhibitor, FR180204, limits hepatocyte apoptosis and reduces DENV-induced liver injury. Clinical parameters, including leucopenia, thrombocytopenia, transaminases and histology, show improvements after FR180204 treatment. The expression of cell death genes was further identified using real-time PCR array and Western blot analysis. Caspase-3 was significantly decreased in FR180204 treated DENV-infected mice compared to the levels of untreated DENV-infected mice suggesting the role of ERK1/2 signaling in immune-mediated liver injury during DENV infection.
UR - https://www.scopus.com/pages/publications/84903708637
UR - https://www.scopus.com/pages/publications/84903708637#tab=citedBy
U2 - 10.1016/j.virusres.2014.03.025
DO - 10.1016/j.virusres.2014.03.025
M3 - Article
C2 - 24704674
AN - SCOPUS:84903708637
SN - 0168-1702
VL - 188
SP - 15
EP - 26
JO - Virus Research
JF - Virus Research
ER -
