TY - JOUR
T1 - Role of interleukin-22 in tuberculosis patients
AU - Gupta, Shruti
AU - Banerjee, Mithu
AU - Gauba, Kavya
AU - Mitra, Prasenjit
AU - Shekhawat, Jyoti
AU - Chauhan, Nishant
AU - Borana, Hemant
AU - Rao, Mahadev
AU - Yadav, Dharmveer
AU - Sharma, Praveen
N1 - Funding Information:
Research funding: The study was funded by All India Institute of Medical Sciences, Jodhpur, India.
Publisher Copyright:
© 2022 Walter de Gruyter GmbH, Berlin/Boston.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Objectives: Disease progression of tuberculosis (TB) depends on the balance between the microorganism's virulence and the host defense systems (mainly T cell-mediated immune response). Interleukin-22 (IL-22) helps in cell proliferation and regeneration and provides protection against microbial diseases. The IL-22-producing T cells can migrate into the granulomas during TB infection. However, disparity exists in literature regarding its role. The present study aims to compare serum IL-22 levels and its' expression in TB patients and healthy controls. Methods: 87 TB patients and 85 healthy subjects were enrolled in the study. Under aseptic conditions, venous blood was withdrawn. Serum IL-22 levels were estimated using enzyme-linked immunosorbent assay, and its gene expression was assessed using SYBR green-based quantitative PCR technology. A statistical analysis was performed using SPSS. Results: The median (interquartile range) of serum IL-22 levels was significantly lower in TB patients (18.55 (5.08) pg/mL) when compared to controls (49.38 (162.88) pg/mL) (p<0.0001). The IL-22 expression was significantly upregulated with a fold change value of 29.44 in TB patients. Conclusions: The IL-22 levels were found to be significantly decreased in patients, contradictory to its expression, which is upregulated. It plays a crucial role for the modulation of tissues in response to TB infection.
AB - Objectives: Disease progression of tuberculosis (TB) depends on the balance between the microorganism's virulence and the host defense systems (mainly T cell-mediated immune response). Interleukin-22 (IL-22) helps in cell proliferation and regeneration and provides protection against microbial diseases. The IL-22-producing T cells can migrate into the granulomas during TB infection. However, disparity exists in literature regarding its role. The present study aims to compare serum IL-22 levels and its' expression in TB patients and healthy controls. Methods: 87 TB patients and 85 healthy subjects were enrolled in the study. Under aseptic conditions, venous blood was withdrawn. Serum IL-22 levels were estimated using enzyme-linked immunosorbent assay, and its gene expression was assessed using SYBR green-based quantitative PCR technology. A statistical analysis was performed using SPSS. Results: The median (interquartile range) of serum IL-22 levels was significantly lower in TB patients (18.55 (5.08) pg/mL) when compared to controls (49.38 (162.88) pg/mL) (p<0.0001). The IL-22 expression was significantly upregulated with a fold change value of 29.44 in TB patients. Conclusions: The IL-22 levels were found to be significantly decreased in patients, contradictory to its expression, which is upregulated. It plays a crucial role for the modulation of tissues in response to TB infection.
UR - http://www.scopus.com/inward/record.url?scp=85140626947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85140626947&partnerID=8YFLogxK
U2 - 10.1515/jbcpp-2022-0106
DO - 10.1515/jbcpp-2022-0106
M3 - Article
C2 - 36239667
AN - SCOPUS:85140626947
SN - 0792-6855
VL - 34
SP - 83
EP - 89
JO - Journal of Basic and Clinical Physiology and Pharmacology
JF - Journal of Basic and Clinical Physiology and Pharmacology
IS - 1
ER -
